LOCUS       Exported                3603 bp DNA     linear   UNA 13-OCT-2022
DEFINITION  apolipoprotein E [Source:HGNC Symbol;Acc:HGNC:613].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     gene ENSG00000130203
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
     gene            1..3603
                     /label=APOE
                     /note="gene ENSG00000130203
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..3154
                     /label=APOE-204
                     /note="primary transcript ENST00000446996"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 6..3603
                     /label=APOE-201
                     /note="primary transcript ENST00000252486"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 6..1536
                     /label=APOE-205
                     /note="primary transcript ENST00000485628
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 22..3235
                     /label=APOE-203
                     /note="primary transcript ENST00000434152"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(79..133,812..877,1970..2162,2743..3235)
                     /codon_start=1
                     /label=APOE-203
                     /note="coding sequence ENSP00000413653"
                     /note="This feature has 4 segments:
                        1:   79 ..  133 / #993366 / ENSE00001601606
                        2:  812 ..  877 / #993366 / ENSE00003463686
                        3: 1970 .. 2162 / #993366 / ENSE00000893952
                        4: 2743 .. 3235 / #993366 / ENSE00001700162"
                     /translation="MSSGASRKSWDPGNPWPPDWPITGRKMKVLWAALLVTFLAGCQAK
                     VEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQEL
                     RALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYR
                     GEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIR
                     ERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRT"
     prim_transcript 570..3164
                     /label=APOE-202
                     /note="primary transcript ENST00000425718"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(835..877,1970..2162,2743..3460)
                     /codon_start=1
                     /label=APOE-201
                     /note="coding sequence ENSP00000252486"
                     /note="This feature has 3 segments:
                        1:  835 ..  877 / #993366 / ENSE00003577086
                        2: 1970 .. 2162 / #993366 / ENSE00000893952
                        3: 2743 .. 3460 / #993366 / ENSE00000893954"
                     /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE
                     LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA
                     EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR
                     KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQ
                     ERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKS
                     WFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH"
     CDS             join(835..877,1970..2162,2743..3164)
                     /codon_start=1
                     /label=APOE-202
                     /note="coding sequence ENSP00000410423"
                     /note="This feature has 3 segments:
                        1:  835 ..  877 / #993366 / ENSE00001620702
                        2: 1970 .. 2162 / #993366 / ENSE00000893952
                        3: 2743 .. 3164 / #993366 / ENSE00001599675"
                     /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE
                     LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA
                     EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR
                     KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQ"
     CDS             join(835..877,1970..2162,2743..3154)
                     /codon_start=1
                     /label=APOE-204
                     /note="coding sequence ENSP00000413135"
                     /note="This feature has 3 segments:
                        1:  835 ..  877 / #993366 / ENSE00001667751
                        2: 1970 .. 2162 / #993366 / ENSE00000893952
                        3: 2743 .. 3154 / #993366 / ENSE00001664168"
                     /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE
                     LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA
                     EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR
                     KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSL"
     primer_bind     2064..2088
                     /label=PCR Forward
                     /note="color: black; sequence: GGCACTGGGTCGCTTTTGGGATTAC; 
                     added: 2022-10-14"
     primer_bind     2879..2896
                     /label=Sanger Sequencing
                     /note="color: black; sequence: GACATGGAGGACGTGTGC; added: 
                     2022-10-14"
     misc_feature    2998..3097
                     /label=Donor Template WT -> SNV
                     /note="color: #00ffff"
     primer_bind     complement(2998..3097)
                     /label=Donor Template SNV -> REV
                     /note="color: black; sequence: 
                     CTCGCGGATGGCGCTGAGGCCGCGCTCGGCGCCCTCGCGGGCCCCGGCCTGGTACACTG
                     CCAGGCGCTTCTGCAGGTCATCGGCATCGCGGAGGAGCCGC; added: 
                     2022-10-14"
     misc_feature    3016..3035
                     /label=Protospacer Sequence
                     /note="color: #ff0000"
     primer_bind     3016..3035
                     /label=gRNA Protospacer
                     /note="color: black; sequence: CGATGACCTGCAGAAGTGCC; added:
                     2022-10-14"
     misc_feature    3032
                     /label=SNV
                     /note="
                     SNV = T
                     REV = C"
                     /note="color: #ff9900"
     misc_feature    3036..3038
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(3447..3471)
                     /label=PCR Reverse
                     /note="color: black; sequence: GGCTTCGGCGTTCAGTGATTGTCGC; 
                     added: 2022-10-14"
     gene            3585..9178
                     /note="gene ENSG00000280087
                     TEC"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3585..9178
                     /note="primary transcript ENST00000623895
                     TEC"
                     /note="color: #a6acb3; direction: RIGHT"
ORIGIN
        1 gagtcctact cagccccagc ggaggtgaag gacgtccttc cccaggagcc ggtgagaagc
       61 gcagtcgggg gcacggggat gagctcaggg gcctctagaa agagctggga ccctgggaac
      121 ccctggcctc caggtagtct caggagagct actcggggtc gggcttgggg agaggaggag
      181 cgggggtgag gcaagcagca ggggactgga cctgggaagg gctgggcagc agagacgacc
      241 cgacccgcta gaaggtgggg tggggagagc agctggactg ggatgtaagc catagcagga
      301 ctccacgagt tgtcactatc atttatcgag cacctactgg gtgtccccag tgtcctcaga
      361 tctccataac tggggagcca ggggcagcga cacggtagct agccgtcgat tggagaactt
      421 taaaatgagg actgaattag ctcataaatg gaacacggcg cttaactgtg aggttggagc
      481 ttagaatgtg aagggagaat gaggaatgcg agactgggac tgagatggaa ccggcggtgg
      541 ggagggggtg gggggatgga atttgaaccc cgggagagga agatggaatt ttctatggag
      601 gccgacctgg ggatggggag ataagagaag accaggaggg agttaaatag ggaatgggtt
      661 gggggcggct tggtaaatgt gctgggatta ggctgttgca gataatgcaa caaggcttgg
      721 aaggctaacc tggggtgagg ccgggttggg gccgggctgg gggtgggagg agtcctcact
      781 ggcggttgat tgacagtttc tccttcccca gactggccaa tcacaggcag gaagatgaag
      841 gttctgtggg ctgcgttgct ggtcacattc ctggcaggta tgggggcggg gcttgctcgg
      901 ttccccccgc tcctccccct ctcatcctca cctcaacctc ctggccccat tcaggcagac
      961 cctgggcccc ctcttctgag gcttctgtgc tgcttcctgg ctctgaacag cgatttgacg
     1021 ctctctgggc ctcggtttcc cccatccttg agataggagt tagaagttgt tttgttgttg
     1081 ttgtttgttg ttgttgtttt gtttttttga gatgaagtct cgctctgtcg cccaggctgg
     1141 agtgcagtgg cgggatctcg gctcactgca agctccgcct cccaggtcca cgccattctc
     1201 ctgcctcagc ctcccaagta gctgggacta caggcacatg ccaccacacc cgactaactt
     1261 ttttgtattt tcagtagaga cggggtttca ccatgttggc caggctggtc tggaactcct
     1321 gacctcaggt gatctgcccg tttcgatctc ccaaagtgct gggattacag gcgtgagcca
     1381 ccgcacctgg ctgggagtta gaggtttcta atgcattgca ggcagatagt gaataccaga
     1441 cacggggcag ctgtgatctt tattctccat cacccccaca cagccctgcc tggggcacac
     1501 aaggacactc aatacatgct tttccgctgg gcgcggtggc tcacccctgt aatcccagca
     1561 ctttgggagg ccaaggtggg aggatcactt gagcccagga gttcaacacc agcctgggca
     1621 acatagtgag accctgtctc tactaaaaat acaaaaatta gccaggcatg gtgccacaca
     1681 cctgtgctct cagctactca ggaggctgag gcaggaggat cgcttgagcc cagaaggtca
     1741 aggttgcagt gaaccatgtt caggccgctg cactccagcc tgggtgacag agcaagaccc
     1801 tgtttataaa tacataatgc tttccaagtg attaaaccga ctcccccctc accctgccca
     1861 ccatggctcc aaagaagcat ttgtggagca ccttctgtgt gcccctaggt actagatgcc
     1921 tggacggggt cagaaggacc ctgacccacc ttgaacttgt tccacacagg atgccaggcc
     1981 aaggtggagc aagcggtgga gacagagccg gagcccgagc tgcgccagca gaccgagtgg
     2041 cagagcggcc agcgctggga actggcactg ggtcgctttt gggattacct gcgctgggtg
     2101 cagacactgt ctgagcaggt gcaggaggag ctgctcagct cccaggtcac ccaggaactg
     2161 aggtgagtgt ccccatcctg gcccttgacc ctcctggtgg gcggctatac ctccccaggt
     2221 ccaggtttca ttctgcccct gtcgctaagt cttggggggc ctgggtctct gctggttcta
     2281 gcttcctctt cccatttctg actcctggct ttagctctct ggaattctct ctctcagctt
     2341 tgtctctctc tcttcccttc tgactcagtc tctcacactc gtcctggctc tgtctctgtc
     2401 cttccctagc tcttttatat agagacagag agatggggtc tcactgtgtt gcccaggctg
     2461 gtcttgaact tctgggctca agcgatcctc ccgcctcggc ctcccaaagt gctgggatta
     2521 gaggcatgag ccaccttgcc cggcctccta gctccttctt cgtctctgcc tctgccctct
     2581 gcatctgctc tctgcatctg tctctgtctc cttctctcgg cctctgcccc gttccttctc
     2641 tccctcttgg gtctctctgg ctcatcccca tctcgcccgc cccatcccag cccttctccc
     2701 cgcctcccac tgtgcgacac cctcccgccc tctcggccgc agggcgctga tggacgagac
     2761 catgaaggag ttgaaggcct acaaatcgga actggaggaa caactgaccc cggtggcgga
     2821 ggagacgcgg gcacggctgt ccaaggagct gcaggcggcg caggcccggc tgggcgcgga
     2881 catggaggac gtgtgcggcc gcctggtgca gtaccgcggc gaggtgcagg ccatgctcgg
     2941 ccagagcacc gaggagctgc gggtgcgcct cgcctcccac ctgcgcaagc tgcgtaagcg
     3001 gctcctccgc gatgccgatg acctgcagaa gcgcctggca gtgtaccagg ccggggcccg
     3061 cgagggcgcc gagcgcggcc tcagcgccat ccgcgagcgc ctggggcccc tggtggaaca
     3121 gggccgcgtg cgggccgcca ctgtgggctc cctggccggc cagccgctac aggagcgggc
     3181 ccaggcctgg ggcgagcggc tgcgcgcgcg gatggaggag atgggcagcc ggacccgcga
     3241 ccgcctggac gaggtgaagg agcaggtggc ggaggtgcgc gccaagctgg aggagcaggc
     3301 ccagcagata cgcctgcagg ccgaggcctt ccaggcccgc ctcaagagct ggttcgagcc
     3361 cctggtggaa gacatgcagc gccagtgggc cgggctggtg gagaaggtgc aggctgccgt
     3421 gggcaccagc gccgcccctg tgcccagcga caatcactga acgccgaagc ctgcagccat
     3481 gcgaccccac gccaccccgt gcctcctgcc tccgcgcagc ctgcagcggg agaccctgtc
     3541 cccgccccag ccgtcctcct ggggtggacc ctagtttaat aaagattcac caagtttcac
     3601 gca
//
