LOCUS       Exported                6257 bp DNA     linear   UNA 20-JAN-2023
DEFINITION  RAB39B, member RAS oncogene family [Source:HGNC 
            Symbol;Acc:HGNC:16499].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2023 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     gene ENSG00000155961
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
     gene            1..6257
                     /label=RAB39B
                     /note="gene ENSG00000155961
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..6257
                     /label=RAB39B-201
                     /note="primary transcript ENST00000369454"
                     /note="color: #ffcc00; direction: RIGHT"
     CDS             join(204..418,3263..3689)
                     /codon_start=1
                     /label=RAB39B-201
                     /note="coding sequence ENSP00000358466"
                     /note="This feature has 2 segments:
                        1:  204 ..  418 / #993366 / ENSE00001450069
                        2: 3263 .. 3689 / #993366 / ENSE00001450068"
                     /translation="MEAIWLYQFRLIVIGDSTVGKSCLIRRFTEGRFAQVSDPTVGVDF
                     FSRLVEIEPGKRIKLQIWDTAGQERFRSITRAYYRNSVGGLLLFDITNRRSFQNVHEWL
                     EETKVHVQPYQIVFVLVGHKCDLDTQRQVTRHEAEKLAAAYGMKYIETSARDAINVEKA
                     FTDLTRDIYELVKRGEITIQEGWEGVKSGFVPNVVHSSEEVVKSERRCLC"
     primer_bind     3033..3057
                     /label=PCR Forward
                     /note="color: black; sequence: GAACATTCGTTTCTAGAAGATGCCC; 
                     added: 2023-01-20"
     primer_bind     3405..3423
                     /label=Sanger Sequencing
                     /note="color: black; sequence: CTGGTGGGTCACAAGTGTG; added: 
                     2023-01-20"
     primer_bind     3486..3585
                     /label=Donor Template SNV -> REV
                     /note="color: black; sequence: 
                     ATGAAGTACATTGAAACGTCAGCCCGAGATGCCATTAATGTGGAGAAAGCCTTCACAGA
                     CCTGACAAGAGACATATATGAGCTGGTTAAAAGGGGGGAGA; added: 
                     2023-01-20"
     misc_feature    3545..3547
                     /label=PAM
                     /note="color: #ffff00"
     misc_feature    3548..3567
                     /label=Protospacer Sequence
                     /note="color: #ff0000"
     misc_feature    3550
                     /label=SNV
                     /note="REV = C
                     SNV = A"
                     /note="color: #ffcc00"
     primer_bind     complement(3551..3567)
                     /label=gRNA Protospacer
                     /note="color: black; sequence: GCTCATATATGTCTCTTTTC; added:
                     2023-01-20"
     primer_bind     complement(3996..4020)
                     /label=PCR Reverse
                     /note="color: black; sequence: TCCTGTCTGTAGGTATGTTTTCCTG; 
                     added: 2023-01-20"
ORIGIN
        1 gctgccattt catcaccttt gcgagcgcag catccatccc tccgctctcc cggcgcctgg
       61 gcctacccag cttcgggctc ccaggccagc gatgcgctcg cggctgagct agatcctgcc
      121 gagccgcgct ctctgaggcg tcggcggggc gccccctccc gccgtccccg gtccgggcca
      181 aggagacctg cagagccgcg gccatggagg ccatctggct gtaccagttc cggctcattg
      241 tcatcgggga ttccacagtg ggcaagtcct gcctgatccg ccgcttcacc gagggtcgct
      301 ttgcccaggt ttctgacccc accgtggggg tggatttttt ctcccgcttg gtggagatcg
      361 agccaggaaa acgcatcaag ctccagatct gggataccgc gggtcaagag aggttcaggt
      421 gggagcgcag tccgggcccg caagcagtgg gagggtcttc ggggtttgca ccgcctctgg
      481 ggagagagga cttcctggtt ctaggtgcgg tccaggaggg caacaccaag ggtcttgagg
      541 attctgccga agcccacctt cggctaaccc ggagtttagt gcaaggaaag aagcgccctg
      601 gcagggaagg ccggtcaata acacatattg cttgcaccac aactccacca acctgccctt
      661 tggtggctaa caaactagct ggaattgatc tctgtatctg taattcatca atctcagtcc
      721 ctatgttaca tactttcaaa aacgttccca ggtcatccta acataactga cagagattta
      781 aatacataaa tacatgtgta ttatgtttta tttccacatc tgtgtgtgtg cacatatata
      841 tgctaacata tgttctaaca tgaaatagat acgattgatt ccttcttgca accagcacac
      901 attcagtgat catctactat gtgccagact ctgggcttgt tcctgggaaa acagtgtgag
      961 gagactcaca caaggggatg ccatgtaatc taaacagaca aagccccttc attcttggaa
     1021 cttctgtgct aacatagttg agggaaatag ataatcagca attacgaaag tgtgtcttgc
     1081 tcatatttgt atgcccactc cctactgtaa cacttggcac aaaatgaaca tgcttaattt
     1141 caatcaatga attcattgaa taatcaatga atatgtttac tttcactcag aaccctttgc
     1201 ttttctattg tagcatgtat tatttgtaat tatatgtatg aacacagagg aggaaaggtt
     1261 aactggagaa gatgagaccg gatgagtatg actgcctaag caaagacagg aaacgcccag
     1321 ggataggaaa cagcatgaat aaagacagaa gtgagaggga gcagagtgta cacagaaaac
     1381 tttgcagaga acaatttgaa ctaaagtaga gcagcaggaa ccagatcaag aaaactcaat
     1441 agcccaagtt tatgatcatt tactcattta tctattgaaa actaactaag catagaagat
     1501 actggttggg taattgaaga ggggtaagga gaagaaataa ttacaaactt aagttacctc
     1561 cagctcccca cttccacaaa caccactgtg gaaaatatcc ttgtacaggt tcactttgaa
     1621 cttgcgattg catttctcct aagcaaagaa aggtatcttt tatgtgtttg ttggaatttc
     1681 tttctatatg aacagcctgt tcatatccac tgcttattta tttattgggc aatatggact
     1741 tgtaagagta ctttatagat taagaatatt aaccctttcc tgtcatgtat gttgtaagat
     1801 atattcctag tttatcatct gtcttttcac attatttaag attttttaat gtagggaaga
     1861 atttaaggtt tatgtagcct tatgtccatg cttagaaggc ctttgtcact ttctttgcta
     1921 agtactcaca aacaattaat gtaataatat attccagagt tttgacagtg atctacagct
     1981 tgcttgttga tttcttgaat caaccttctc acaaatcaaa acctttgttt gttcatgtct
     2041 aaattttctg gtaaatctac cattctccat gaatcttcaa acctaagaga cgagaaatgc
     2101 tttcagtccc cagggctgat ttgcctgatt ttggagactt aaagtcattt gtaagtttta
     2161 cttttaccat catctagctt ggactgtagg tccctggaca atgcttgttc tgctaacatt
     2221 gagccatttt ctctaagcta ttgagtcctt cgttgtctat cttctttatc tgaggagaca
     2281 gaaaacctaa aaatagacat aggtaaaaaa aatcacagta gtctaaagca agtgactgca
     2341 gagacaaata ggtccatacc ctgtaataag ctttaataca ctgtaagcaa agtgccataa
     2401 tgtatattta taatgaggtt cctcaagtca agaagttaaa acatgtgaag tttcttctga
     2461 tggctcctga gttgagttgg cctctctgaa atgtgtcagg aaacagggct gagaggcact
     2521 ttgcctgaac ccttcgccat ctctcttttt cccattccca caggccttga aaataaaaaa
     2581 taaaattgtg tttctcctca cagaagagat tgaggatccc tgttagctaa taataataat
     2641 aataataata ataaacaata ataataatgg aaagagaaaa aaacatggta atgataaatg
     2701 gaaaaaatgg agtagcaaga tacctacttt aaatattttg ttaactatca taaattcatt
     2761 tctatgtttt aaaaatcctg tttcaactgc tagcatttgt aatgaactct ccacgtcaca
     2821 gaacacttta atatccattg tttcattttg cctcatggta acactgtgac ttgggtatcc
     2881 tattttgcag aaaaggaagt ctctgtcagt tattcagatt tggggggtgg gagagttagt
     2941 tgtagtttta cctgaaggca agaaatagaa ctcagttatg tcccagggtc actctccagt
     3001 gctgaatcca tggaaaatgc ctaaaagttc atgaacattc gtttctagaa gatgcccatc
     3061 tcaaagtttg gcccaattgg aaactcacat attagtagta gtatggaatg ccatttgtat
     3121 tcctttgttt ttttaaaaaa aaagaaaatc atttttatta aaggactgtg tcactaacca
     3181 ggaacacttg aatatactga gttcattgac atgcagaagt ttgggcacag cctttactct
     3241 ttacttcttt cttgtgtttt agatccatca ctcgcgccta ctacaggaac tcagtaggtg
     3301 gtcttctctt atttgacatt accaaccgca ggtccttcca gaatgtccat gagtggttag
     3361 aagagaccaa agtacacgtt cagccctacc aaattgtatt tgttctggtg ggtcacaagt
     3421 gtgacctgga tacacagagg caagtgactc gccacgaggc cgagaaactg gctgctgcat
     3481 acggcatgaa gtacattgaa acgtcagccc gagatgccat taatgtggag aaagccttca
     3541 cagacctgac aagagacata tatgagctgg ttaaaagggg ggagattaca atccaggagg
     3601 gctgggaagg ggtgaagagt ggatttgtac caaatgtggt tcactcttca gaagaggttg
     3661 tcaaatcaga gaggagatgt ttgtgctagt cagttctttt atttccaaaa catgctctcc
     3721 tacttgaact gaaaagtaag agaaataaat agaatctttg tgtaactgat gagaactcaa
     3781 cctccatttg gataccaagt gagcctccct ctcaggggga ctctgacaga ctcgtgtgcg
     3841 ggtggatggg ggtgcctgag tgctgtgacc aagggactga attgctgaca tgggccaggc
     3901 caggcactgt tttgtggccc atgctaggca gtggctgctc catgagccct ttgtggacca
     3961 catgttaaag gcctggggtt taggaagcta agtcccagga aaacatacct acagacagga
     4021 caactgttcc ttggtatttg agctcactta taggccatga acataaagaa atacataaag
     4081 gggaaaaaag tttcacaatg gggacaatta gcataactaa acaaagatta tgagctctac
     4141 ttattccgtg agaataccct gcaccaaagg tgtttgctgg gtttggttaa aaggtaccaa
     4201 ttagctttta acagctttct gtaaaatgag gaacacagac caaaaggtct ggtgttcaag
     4261 taaaaatgat gtgactatgg aaaggaaggc aatcgacaca gttattagca catttcagaa
     4321 aatgaacacc cttgtgatag aattaccagc tgtatgaaac aaattgtccc atgcaaatta
     4381 ccctataact aggaaagaga cactataacc caaatgccat accccctttt tcctttgacc
     4441 atgcctgggg tttcaatgcc ttggcacctg caatctggaa gtaaacagtc tgcttgcttc
     4501 ttcaggcacg tgacagtgac aacacagtga ctgcctctga aaagagccct gaggcaaaca
     4561 ctctctcctg ctgaacagga ggcactgtaa cccttacaaa cagcttacag ttacttgggc
     4621 tacttatttg atcatcataa caggcctacg aggtttttgc cccattttac agaatagtaa
     4681 actaagagcc atgaaaatca cttggccagg gtcaaggact cgtgactcct agctcttttc
     4741 actgcatcat actgctgtca gatgaataaa tgattatgaa tttgtttact tgggaagaga
     4801 tatgactata aataaatgtt ctctacatgt taggatatgt tttccttatt gattacaact
     4861 cagctataat cctgagggaa catgggaagg aatcaaaggt aaagcccatt caggcattat
     4921 tgacaagagg gctttaaaca agtgaataat tgcacaacta aaaagacatt agaactgtgt
     4981 gggctataag tgttcttatc actgagacac agtggatttt tattgaaagg aaccacgtac
     5041 agcaatgcgg agtgggatag ggataaaata cccaccccaa aatgaccttg gccacctgtc
     5101 tgccatatac gtacactgag ttcctgtcta cccaagagag tcagccctta attctcaggt
     5161 gaacaaacag ctaactgcat cagcaaattg gaatggctgg cttgaagttt ccattatttt
     5221 cttgaagaga ggtgatgctt taaattactt tttggattgt agccagttca gctaggcaaa
     5281 agccatactt ttcacaatta aaaaaaaaaa ctattctgac tagagtcagg tttctcatat
     5341 ccattgtgat aggaaggcct aagaatggca aatcgctttg agatataata ttgaacttca
     5401 gtatgaatgt gaccgaatct gaaactttgg gaatttgctt tagccagaat aaagaaacca
     5461 aatcatgaaa attatggaag actattctgc cacctgtgcc ccaagttgga tgagcaaacc
     5521 actttggatc agttcacagc attaactaca agtgaactga tcatcttctc tatattcatt
     5581 tcacaagagc tggcttaggt ggggtaatta aattgccacc ttcgtggttg taaaagacaa
     5641 acaaaacatg gcaaatttca ttaacacaaa atttcatcaa ctgctatact gaattatgtt
     5701 tagaagattc tgaaagcaag attatccagt gggtaaatga gagtcgactg gattctgtca
     5761 gttcaaaaaa ggaaagaaac aaaacttccc tacaattttc ttcactaaaa tttattatat
     5821 gtacagtatt cccactcctg gcattttctg gcaggtttaa aaatattcag cataaatcca
     5881 gcagaggtgg ctgagcttaa ttgcttaaat aagttctaga aaatttaaaa atcattttag
     5941 tagaattcag taccattgtc actgcaataa gtgggtttta gaaaataagt gtgaaaagaa
     6001 tcaggtgtta atataatact aaggataaag ctttagaagc tattttacta cataggtaaa
     6061 gaaaagataa actaacttgt aacaaagacc acaactgcat gttggattag attgtctcat
     6121 atttcagaat aaaatgtact gtatactttt cctcactttg ttgtgcactg taatgaaatg
     6181 tgaaaaatgc tttatttagc tgtatgtgaa tgaaaatatg cttgtattta gtaaaatgat
     6241 tgattatgtg actgtga
//
