LOCUS       INK2J00086R_UBQL        4242 bp DNA     linear   UNA 17-AUG-2023
DEFINITION  ubiquilin 2 [Source:HGNC Symbol;Acc:HGNC:12509].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000188021
FEATURES             Location/Qualifiers
     source          1..4242
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..4242
                     /label=UBQLN2
                     /note="gene ENSG00000188021
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..4242
                     /label=UBQLN2-201
                     /note="primary transcript ENST00000338222"
                     /note="color: #ffcc00; direction: RIGHT"
     CDS             248..2122
                     /codon_start=1
                     /label=UBQLN2-201
                     /note="coding sequence ENSP00000345195"
                     /note="color: #993366"
                     /translation="MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEE
                     FAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKS
                     QNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTN
                     FSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEIS
                     HLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLN
                     AAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGS
                     GSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSL
                     SQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQI
                     QQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIG
                     PTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLP
                     NPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS"
     primer_bind     1554..1578
                     /label=PCR Forward
                     /note="color: black; sequence: AGATGCAGAATCCAGACACACTATC; 
                     added: 2023-08-15"
     primer_bind     1579..1598
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: AGCCATGTCAAACCCAAGAG; added:
                     2023-08-15"
     misc_feature    1703..1802
                     /label=DNA Donor Template Sequence SNV -> Rev
                     /note="color: #00ccff"
     primer_bind     complement(1703..1802)
                     /label=DNA Donor Template SNV -> Rev
                     /note="color: black; sequence: 
                     TGGGTCCTATGGGCCCAATGGGGCCTATGGGGGTAAAAGGGACTATAGGGCCTATGGGG
                     CCTATGGGGGTGACTGGGCCTACAGGGCCTATAGCGGTTCC; added: 
                     2023-08-15"
     misc_feature    1721..1740
                     /label=gRNA Protospacer Sequence
                     /note="color: #ff0000"
     primer_bind     1721..1740
                     /label=gRNA Protospacer
                     /note="color: black; sequence: GTAGGCCCAGTCACCCACAT; added:
                     2023-08-15"
     misc_feature    1737
                     /label=SNV
                     /note="SNV = A
                     REV = C"
                     /note="color: #ff9900"
     misc_feature    1741..1743
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(2113..2137)
                     /label=PCR Reverse
                     /note="color: black; sequence: GGTACAGAAATGTGATTACGATGGC; 
                     added: 2023-08-15"
ORIGIN
        1 acaaggcggc ggcggaggag gcccagagac cggagcgcgg agacctcagc cagcggccta
       61 cgcccaggcc tttctccacc ggaggaccag ggaaccgcag tcttcatcac agaggtaccg
      121 tgctccgcgc tccccgcctg acccggccca gcccgctgcg gcggtgcctc cttccttcct
      181 ccttccctcg cgctctctct ttcgcccgcc cgcgccttcc ctgcccgcct gcgtcaccgc
      241 ggccgccatg gctgagaatg gcgagagcag cggccccccg cgcccctccc gcggccctgc
      301 tgcggcccaa ggctcggctg ctgccccggc tgagcctaaa atcatcaaag tcacggtgaa
      361 gactcccaaa gagaaagagg agttcgcggt gcccgagaac agctcggttc agcagtttaa
      421 ggaagcgatt tcgaaacgct tcaaatccca aaccgatcag ctagtgctga tttttgccgg
      481 aaaaatctta aaagatcaag ataccttgat ccagcatggc atccatgatg ggctgactgt
      541 tcaccttgtc atcaaaagcc agaaccgacc tcagggccag tccacgcagc ctagcaatgc
      601 cgcgggaact aacactacct cggcgtcgac tcccaggagt aactccacac ctatttccac
      661 aaatagcaac ccgtttgggt tggggagcct gggaggactt gcaggcctta gcagcctggg
      721 cttgagctcg accaacttct ctgagctcca gagccagatg cagcagcagc ttatggccag
      781 ccctgagatg atgatccaaa taatggaaaa tccctttgtt cagagcatgc tttcgaatcc
      841 cgatctgatg aggcagctca ttatggctaa tccacagatg cagcaattga ttcagagaaa
      901 cccagaaatc agtcacctgc tcaacaaccc agacataatg aggcagacac tcgaaattgc
      961 caggaatcca gccatgatgc aagagatgat gagaaatcaa gacctggctc ttagcaatct
     1021 agaaagcatc ccaggtggct ataatgcttt acggcgcatg tacactgaca ttcaagagcc
     1081 gatgctgaat gccgcacaag agcagtttgg gggtaatcca tttgcctccg tggggagtag
     1141 ttcctcctct ggggaaggta cgcagccttc ccgcacagaa aatcgcgatc cactacccaa
     1201 tccatgggca ccaccgccag ctacccagag ttctgcaact accagcacga ccacaagcac
     1261 tggtagtggg tctggcaata gttccagcaa tgctactggg aacaccgttg ctgccgctaa
     1321 ttatgtcgcc agcatcttta gtaccccagg catgcagagc ctgctgcaac agataactga
     1381 aaacccccag ctgattcaga atatgctgtc ggcgccctac atgagaagca tgatgcagtc
     1441 gctgagccag aatccagatt tggctgcaca gatgatgctg aatagcccgc tgtttactgc
     1501 aaatcctcag ctgcaggagc agatgcggcc acagctccca gccttcctgc agcagatgca
     1561 gaatccagac acactatcag ccatgtcaaa cccaagagca atgcaggctt taatgcagat
     1621 ccagcagggg ctacagacat tagccactga agcacctggc ctgattccga gcttcactcc
     1681 aggtgtgggg gtgggggtgc tgggaaccgc tataggccct gtaggcccag tcacccccat
     1741 aggccccata ggccctatag tcccttttac ccccataggc cccattgggc ccataggacc
     1801 cactggccct gcagcccccc ctggctccac cggctctggt ggccccacgg ggcctactgt
     1861 gtccagcgct gcacctagtg aaaccacgag tcctacatca gaatctggac ccaaccagca
     1921 gttcattcag caaatggtgc aggccctggc tggagcaaat gctccacagc tgccgaatcc
     1981 agaagtcaga tttcagcaac aactggaaca gctcaacgca atggggttct taaaccgtga
     2041 agcaaacttg caggccctaa tagcaacagg aggcgacatc aatgcagcca ttgaaaggct
     2101 gctgggctcc cagccatcgt aatcacattt ctgtacctgg aaaaaaaatg tatcttattt
     2161 ttgataatgg ctcttaaatc tttaaacaca cacacaaaat cgttctttac tttcattttg
     2221 attcttttaa atctgtctag ttgtaagtct aatatgatgc attttaagat ggagtccctc
     2281 cctcctactt ccctcactcc ctttctcctt tgcttatttt tcctaccttc ccttcctctt
     2341 gtctccccac tccctccctc tttgtttcct tccttcctta tttcctttag tttccttcct
     2401 tagccgtttt gagtggtggg aatcaatgct gtttcactca aaagtgttgc atgcaaacac
     2461 ttctctttat tctgcattta ttgtgatttt tggaaacagg tatcaacctt cacagttggg
     2521 tgaacaagtg ttgtcctaca gatgtccaat ttatttgcat ttttaaacat tagcctatga
     2581 tagtaattta atgtagaatg aagatattaa aaacagaagc aaattatttg aagctctcta
     2641 atttgtggta cgatattgct tattgtgact ttggcatgta tttttgctag caaaatgctg
     2701 taagatttat accattgatc ttttttgcta tatttgtata cagtacagta agcacaattg
     2761 gcactgtaca tctaaaaata ttacagtaga atctgagtgt aatatgtgta accaaaatga
     2821 gaaagaatac aagaaatgtt tctggagcta gttatgtctc acaattttgt agaatcttac
     2881 agcatctttg ataaacttct cagtgaaaat gttggctagg caagttcagt taaaatatag
     2941 tagaaatgtt tatcctggta tctctaagta tacatttaat tgtacagaaa atttacagtg
     3001 taacattgtg tcaacatttg cagattgact gtatatgacc ttaatctttg tgcagcctga
     3061 aggatcagtg tagtaatgcc aggaaagtgc tttttaccta agacttcctt ctcagcttct
     3121 cccataaaga gaccctaata tgcattttga tttgtaattg gaaatgtaac tttcactgaa
     3181 agtgtcatgt gatgtttgca ttacttttaa ctgctatgta taaaggaaag tgtgtctttt
     3241 gacttcatca gttatttctc ttgtgcacag agaaaaatgc attaaaaatg actaaaaaaa
     3301 ataaaaaatt aaaaaatgga taaatctttt ctttttgcct tttggcccta ggatcgtgtt
     3361 taggaggatt atcccacccc gagattatat aaatcttatc ctatatttct ctaacttata
     3421 tggttttatt tagaaaatgt tttgtcctgt ctggaattat cttgatgtat ggatttaggt
     3481 attctaactt ttttgcccca aagggttagc cagttgttaa catatttacc ttttccccca
     3541 acatatgaaa tgtcatacat gtatatactt tattctgtgt ttggattccc tttagttcca
     3601 ttgaacattg tggcaccagt acaccagcct gtagatgagt tagaaatggg actttgtatc
     3661 ttttaatgtg agacctcctc ttgatctttt ttatttttta caatattctg acgattctga
     3721 catgtttatt ttaccagatg aattttacag ttaatagatt cttcccccaa aaatatattg
     3781 agaatttggt tgggattctc ttaaattgat atgactgtga ggactacaga attgcattgc
     3841 tttccagaaa catgttgcct tgtttctgaa aaactttata tatccttcaa gagagtttta
     3901 cttaaatcct atataatttg taataaatat gttcctaggt aatacagaca tacagggaac
     3961 acgttgatac tgcttattgg agcttttaat atgttaccta tgtggtcatt tttgctatat
     4021 aggagaagaa tttatttgca tcgattattt taaattggca atttattgaa atctattaat
     4081 tttagagata ttcatttgat ccttttgagt ttttccaggt gagaaatcct atcaattatg
     4141 ttataattat atgttttcct tttcaaaatt tataacataa tttctgactg catgggttaa
     4201 acattttcat aacagtgcta aataataaag gtggcagtgg ca
//
