LOCUS       INK2J00057R_KNCA        8352 bp DNA     linear   UNA 13-JUN-2023
DEFINITION  potassium voltage-gated channel subfamily A member 1 [Source:HGNC 
            Symbol;Acc:HGNC:6218].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000111262
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..8352
                     /label=KCNA1
                     /note="gene ENSG00000111262
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..8352
                     /label=KCNA1-201
                     /note="primary transcript ENST00000382545"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 3..101841
                     /note="primary transcript ENST00000638821
                     lncRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     gene            37..116108
                     /note="gene ENSG00000256654
                     lncRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 37..116108
                     /note="primary transcript ENST00000640877
                     lncRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 201..116086
                     /note="primary transcript ENST00000640962
                     lncRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 464..21457
                     /note="primary transcript ENST00000541095
                     lncRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 464..8282
                     /label=KCNA1-202
                     /note="primary transcript ENST00000543874
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1475..2962
                     /codon_start=1
                     /label=KCNA1-201
                     /note="coding sequence ENSP00000371985"
                     /note="color: #993366"
                     /translation="MTVMSGENVDEASAAPGHPQDGSYPRQADHDDHECCERVVINISG
                     LRFETQLKTLAQFPNTLLGNPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRR
                     PVNVPLDMFSEEIKFYELGEEAMEKFREDEGFIKEEERPLPEKEYQRQVWLLFEYPESS
                     GPARVIAIVSVMVILISIVIFCLETLPELKDDKDFTGTVHRIDNTTVIYNSNIFTDPFF
                     IVETLCIIWFSFELVVRFFACPSKTDFFKNIMNFIDIVAIIPYFITLGTEIAEQEGNQK
                     GEQATSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILF
                     SSAVYFAEAEEAESHFSSIPDAFWWAVVSMTTVGYGDMYPVTIGGKIVGSLCAIAGVLT
                     IALPVPVIVSNFNYFYHRETEGEEQAQLLHVSSPNLASDSDLSRRSSSTMSKSEYMEIE
                     EDMNNSIAHYRQVNIRTANCTTANQNCVNKSKLLTDV"
     prim_transcript 1637..8316
                     /label=KCNA1-203
                     /note="primary transcript ENST00000639306
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2197..2221
                     /label=PCR Forward
                     /note="color: black; sequence: CGCCTGCCCCAGCAAGACGGACTTC; 
                     added: 2023-06-13"
     prim_transcript 2287..8300
                     /label=KCNA1-204
                     /note="primary transcript ENST00000639680"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(2287..2362,3737..3775)
                     /codon_start=1
                     /label=KCNA1-204
                     /note="coding sequence ENSP00000492218"
                     /note="This feature has 2 segments:
                        1: 2287 .. 2362 / #993366 / ENSE00003807784
                        2: 3737 .. 3775 / #993366 / ENSE00003801290"
                     /translation="RDS*AGRKPEGRAGHLPGHPQGHPLGLHSDPWLSELDV"
     primer_bind     2580..2599
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: CCATGACCACTGTAGGATAC; added:
                     2023-06-13"
     misc_feature    2660..2759
                     /label=Donor Template SNV -> REV
                     /note="color: #00ccff"
     primer_bind     2660..2759
                     /label=Donor Template SNV -> REV
                     /note="color: black; sequence: 
                     GGTGTGCTAACAATTGCCCTGCCCGTACCTGTCATTGTGTCCAATTTCAACTATTTCTA
                     CCACCGAGAAACTGAGGGGGAAGAGCAGGCTCAGTTGCTCC; added: 
                     2023-06-13"
     misc_feature    2719..2721
                     /label=PAM
                     /note="color: #ffff00"
     misc_feature    2722..2741
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     misc_feature    2723
                     /label=SNV
                     /note="SNV = T
                     REV = C"
                     /note="color: #ff6600"
     primer_bind     complement(2724..2741)
                     /label=gRNA Protospacer
                     /note="color: black; sequence: CTTCCCCCTCAGTTTCTCAG; added:
                     2023-06-13"
     primer_bind     complement(3112..3136)
                     /label=PCR Reverse
                     /note="color: black; sequence: TTCACCACCCCCAAAATCCTCAATG; 
                     added: 2023-06-13"
ORIGIN
        1 agaaatggac cgagcggacc cgccgccgca cgcaccctgc tccactccaa gctcctaagg
       61 gctcctggcg cgccgcgtag ccttggcgag gtccgcgctg gggtgcggag agcgaaggga
      121 actggagagc catgtagatc caggctctcg cccgcccgcc tccttcggga tcgaatcaag
      181 ggctcccata gtgttaggag ggggcgagag tgctgtttat cgtcatttgc ctcggagctt
      241 cgagagaggg tggtattttg cttttccgcc ccgcatcctc cggaactccc tgcaccggag
      301 agaggacggc gtctccaggt tgctggcaac cggtgagaat gggggtaggg aaggaacatt
      361 ttcgccgtag ctgctccgta aagcgattgt ccaactgaga ggggcgtcgg acgagtggac
      421 cagggcggcg agtttgcccg gcgcgtctcg gatgctgctg cggcggccgc cgcggctccc
      481 gccagggcac tgcaaagacg acctgccgca ttcccactcg ggctctccgc tgactcagca
      541 ccgcccctgc gccaagccag ccggccaggt agggggttcc ccagctcggg gatgcagaag
      601 cgggggttgg ggggaccggg tgggggaggc cgggggtgcg gggatgctgt ccgggaccct
      661 gagcttcccc cggcgtctct cggcgctttt ccgatctcta gtttaacgaa gttgtaaaca
      721 gatcggctgt tgggcattgg ggaaagtggg atggaagagc cccaaacttg gatttccggg
      781 tgtctgcgtg tcgtctgtcc gtgtgtgtgt gatagcccta gcaaacgtcc agtgctttct
      841 caagctagag gtctgtgttc ttcggtgtct gtaggtccgt cccatctgaa tgcttctgat
      901 tttctacccc cgtatcactt tctatttctc tgcagcgtgc atcgatcgcc ctggtgggag
      961 cttagaaggc ggcaggcgaa gaggggtagg aggggggaga gccgaggaga agcagagagg
     1021 gtggcaggcg tggggatctg ccgagccggc actgcaccgg gtcctaggaa ggctctcgga
     1081 ggggagggga ggccagggcg acccccgaag caatggccca gtccgctaga acggcactgc
     1141 gttaaggcac ctgggatcag gaagaaatat ctaaacaaca acaacagaaa accaacaaac
     1201 ccccaaaccc aaacccaacc ctctgcaaaa agctgcaccc ggcccgcagg cgagggggat
     1261 tccaaactga gtgaaaggca gggtggaggg gaaggcagcg agaggcaaag tcgcagatct
     1321 cccgacctgc tcgtgttgaa gcacctcccc ctgggcgtga gggagacgcg cgctccggtg
     1381 ggggggccgc ttgggtcccc cccacccctg gtccctggct gcttcccacc ccgggctctc
     1441 tcctggcctc ccacccccgc gcccggcttc caccatgacg gtgatgtctg gggagaacgt
     1501 ggacgaggct tcggccgccc cgggccaccc ccaggatggc agctaccccc ggcaggccga
     1561 ccacgacgac cacgagtgct gcgagcgcgt ggtgatcaac atctccgggc tgcgcttcga
     1621 gacgcagctc aagaccctgg cgcagttccc caacacgctg ctgggcaacc ctaagaaacg
     1681 catgcgctac ttcgaccccc tgaggaacga gtacttcttc gaccgcaacc ggcccagctt
     1741 cgacgccatc ctctactact accagtccgg cggccgcctg cggaggccgg tcaacgtgcc
     1801 cctggacatg ttctccgagg agatcaagtt ttacgagttg ggcgaggagg ccatggagaa
     1861 gttccgggag gacgagggct tcatcaagga ggaggagcgc cctctgcccg agaaggagta
     1921 ccagcgccag gtgtggctgc tcttcgagta ccccgagagc tcggggcccg ccagggtcat
     1981 cgccatcgtc tccgtcatgg tcatcctcat ctccatcgtc atcttttgcc tggagacgct
     2041 ccccgagctg aaggatgaca aggacttcac gggcaccgtc caccgcatcg acaacaccac
     2101 ggtcatctac aattccaaca tcttcacaga ccccttcttc atcgtggaaa cgctgtgtat
     2161 catctggttc tccttcgagc tggtggtgcg cttcttcgcc tgccccagca agacggactt
     2221 cttcaaaaac atcatgaact tcatagacat tgtggccatc attccttatt tcatcacgct
     2281 gggcaccgag atagctgagc aggaaggaaa ccagaagggc gagcaggcca cctccctggc
     2341 catcctcagg gtcatccgct tggtaagggt ttttagaatc ttcaagctct cccgccactc
     2401 taagggcctc cagatcctgg gccagaccct caaagctagt atgagagagc tagggctgct
     2461 catctttttc ctcttcatcg gggtcatcct gttttctagt gcagtgtact ttgccgaggc
     2521 ggaagaagct gagtcgcact tctccagtat ccccgatgct ttctggtggg cggtggtgtc
     2581 catgaccact gtaggatacg gtgacatgta ccctgtgaca attggaggca agatcgtggg
     2641 ctccttgtgt gccatcgctg gtgtgctaac aattgccctg cccgtacctg tcattgtgtc
     2701 caatttcaac tatttctacc accgagaaac tgagggggaa gagcaggctc agttgctcca
     2761 cgtcagttcc cctaacttag cctctgacag tgacctcagt cgccgcagtt cctctactat
     2821 gagcaagtct gagtacatgg agatcgaaga ggatatgaat aatagcatag cccattatag
     2881 acaggtcaat atcagaactg ccaattgcac cactgctaac caaaactgcg ttaataagag
     2941 caagctactg accgatgttt aaaaaacaaa ggcaagcaaa caaaaaagcc ccacttagca
     3001 gctcaaaaga cttaaaaaac aaaacagaaa acctagtgac tcatgtcacg ctttgtagat
     3061 actttactaa gtagacttgg aatgctctat ttaactgtca atgcgttgtt gcattgagga
     3121 ttttgggggt ggtgaaccag aagctttcaa gatccatgac aaaataaact attttccttt
     3181 tattaaaaaa tgggaaaaga gagagtattt tctaaaactg gcttaaaaag attcagtcca
     3241 cgaactagtc taggtaaaat aataatcata tgcttcccca aactgaaaca tttttaatgc
     3301 tttggtttct ttaacttttt taaaaactca gaacaagatg atcacttaga aatatgaaat
     3361 tgaaattcgc atgggactcc agtaaaacat ctttgcaaac tgcgtagcac attgaagaca
     3421 gtgcatcaga tgtattatat gtaacatgat agaccagcca aaatggacaa tgaatagata
     3481 tttttatttc gatcaactga actgcatatt acaaggtgaa aaaagaaaac tccgattact
     3541 taagactggt tcacaaagca ccttataaat tggatactgg tcctgatctg tagggatttc
     3601 cccctgggcc cattctcttt ctaatccaga ttattctcta agaaaaagtt aactgaatta
     3661 aattaattga ttcttctgca gtgccgctaa atggtctcaa ctgcagatga gccaaataca
     3721 ggtcttttct caccaggcct gcactccgac ccctggcttt cagaactgga tgtaaaacct
     3781 tagcctcctt attgcaagag agcacaaatg aagttaaatg taagcatgtt tgaatctgat
     3841 acaatttatt ttataatcgc atgctgagaa gttaacccag acaatagggg ataagcttaa
     3901 gttgaaatcg attcttctaa aaatagatcc tttttcattt gcattcacca aaagtgcact
     3961 cctccattta ttaactattt tattagtaaa taaagtactg tatttaagtg catatgttag
     4021 tcagatggga acaataactt tttggagctc aaagcatgtt ctcttattca gcattatggc
     4081 ctatttgact aagatgtacc ttgaattaat taatgcatga tttcagtaat aaaaatttta
     4141 aaagtaataa aaattacaag tctgtggggt gaaaggccca atagaaatta tggggggtgg
     4201 gggtgggggc actcagtcaa ttttcctgcc tttgctcagg gaaataccag gtttttgtgc
     4261 aggtataggc ggagagagga ccaatatgcc catcccttaa agggaagcca tgtgaaaaac
     4321 tcaataagtc atcaaagtac atatagcaac acctaagaac aagtattctt tctagctgaa
     4381 gacaaacaca agcaacacaa acaagcaaac aaacaaacaa acaaaaaggt gcaatactgc
     4441 atgttttttg gtgcattctt aggatgtaaa tgaaaatgtt tctctattat atgcatccga
     4501 agcagagctg attttttttt ctttgcagtc attctttgaa gtctgtagag acttcagccc
     4561 tccccttgag gctccctgaa gaaactaaac caattgattt aatagttgct tagtgccttt
     4621 atcctgtacc cacagtgaac tgtagaaagt gcctccttaa cacagctgag aagttaggta
     4681 gcaaaagtgg ggaagggttg gggcacagac cttttgcttt ttctttttcc attctcgctc
     4741 tctcatttca ccactgtgag aagaccacac caccctaaac cctggagagg agagacccag
     4801 gagggtgctg tctctctggc catctactag cattggtccc tttgacagcc tgacgctgga
     4861 tgtgaactga gacccatctt tgaactggac atgaactgtg aacttgtttt ttcctctctc
     4921 caccaaagcc aagataaact ttttgggaat ttgtttccta tcgagggcca ctttggacac
     4981 acaaggcttc ctcaggtcca gtgtagtgct cctggcacct ttccttattt ttttctctgt
     5041 cggtaacagc actttgcaaa tctctctgac ggtccagtct tttcaggcat tgttgtggat
     5101 gtgggaacac tcagttcata ataacctttc ctaggccttc cctcctggtc tacccctttc
     5161 agatatttcc tgatgcccct atgatcttcc cacctggcag tcacttcaca ggttgaacat
     5221 ctaacttctg ctgccccccc gttacccagc ccagagaatg gtggggaccc tgttcctggc
     5281 tgaaagagag ccacggaaca caggcctctg gagctcggca gctgcccacc ggtggagagg
     5341 tactcacagc ctttcaaagg accctgaggt ggggaatctt cattctgcac ttagcatgtg
     5401 gctgcctgtt acctgacatt ctggcccagc ttcttctgaa aatctgtcct gcttctccca
     5461 cccctttccc ccatcctgtc ctagaactag cagtgaggca atcaccctag aaactcgagt
     5521 tacacccatt ctggctaact cgattaaaag aaagaacatg gatatcttat tttcctatgt
     5581 gacttttgtg aatctgtgag atgaacacaa cacacatcgt ggaagatgag gggccaagaa
     5641 ctgcgcagca tccgactaca gggcattaaa ccctcccatg tgatgtctcc ttctcgtctg
     5701 aaccttaact cattctggcg attcctttcc tacttgctta aaaatcccca attaggaaaa
     5761 aaatgccaac ctcctcccag gcttggggtt atcttgatct ttcatttcaa cgttgaggtc
     5821 tagtgcacac agaacttgaa acaccgcact ctgtcaacag caataatcca ctcagtactg
     5881 tgggatggat gggttaatgg atgagaaaac ggcaccaaga ctgactttgg gtatttggta
     5941 tatttaccgt ggttacactc tggactggtt gattcttctg catgtgatat agaaaaaagt
     6001 gctgcatgcg gtgaacctgt cagtctggga ctgggggtga gttgttgtta tgagtttggg
     6061 gtggtgtgag gaaagtgaat gctgcttagc tcatctgtag ctcattgcaa tgaattcagt
     6121 aagaatggag tacagggatt atctgtgtag cataggcatg caatgtttga ccaagctctt
     6181 accctcgcac tgtaatgtgt tgaaatgtct ttgtagacct gaaggtgcac ttaacaaaac
     6241 tgcctattaa gggatgacta ttttttggtt tacttattca tatttatttt agcagccttt
     6301 ttaccttttc ttccccttcc taggcatgga gctgtaacag ctcatgtcct gactatgtgt
     6361 tttctccaga aggagaagac ttctgatgtg ctgatagcta taattcctct cttccagtct
     6421 attggggccc tagttcaata gggtggcaat agaagagttg gtcacaccag ggctgttagc
     6481 catcccagaa tctctgaagt ggttaactca cctgaagtga tctgaatcag agagaccaaa
     6541 gacattcatt tcctctgtcc tcagatttct agaagacaaa ttctagccag gaaaactttc
     6601 tgtttttgca tctccctttt cccatttgtg cccaactccc cttgcactcc ctggagactt
     6661 gagttctgat tttcagttat atcagctcat tgggagtgtg ttcgtgggtg agccttgcag
     6721 aatcagataa accaatagca agtccttctc caggattctg gccagtgggc agtcatttcc
     6781 ctgaaatgaa ttgtagggca ggtttagatt ccactatggt ggacgtgaag cacaggtggt
     6841 gtcacaatct tgatttgctt gagaattaaa aaaatacatg taaatggtaa atgaggaata
     6901 cattttttta aagtagaaat ttggtttagc ctataaaagg ccttcttcac attgtgtata
     6961 gttacacgtt tttcaaatta ggtaccatct aaaagtcaat aaaaatagtt tcctagccct
     7021 ctcatttaat ataggagacc tcaaaacaca tgctttttaa caattttaca ttttaattct
     7081 tagtttggta gattggattg aaagaaagga gaaagaacat tagcagaagg cactttccca
     7141 ttttcttcca ggaatgactt attcctgggg tggtgagaat tggtgggtgg taaccatcca
     7201 tagtataaaa ttgttagaaa gaatataaac tgccaaacaa gcatgttatc ttcaggcttt
     7261 tccaagcaag aatgaagtct tttgatgttt atgttcattt taagaagaca aacaaactaa
     7321 aattttaaga ccagacacaa cccaagttga attgtgatct caggaggtga actctcattc
     7381 accatgtggc atattactac ctgtatttat atcatggaat ttcagggtat atgtgaacat
     7441 gtctagtatg actcaggtaa acctttaaag aatgtatgtt acttaccatt tttgtaaaga
     7501 agcaaacagg agctgaattg ttaaccaaaa ctgttccatt accttgggtc actgtgcaaa
     7561 ctaattcagg gtacagatat aaagtttaga agccttggga ttaaatgcca gcctctttgc
     7621 tcaggcaaaa tgaccctggg ctttcttggg aagtccagca tgtatgtaag gggtgaggcc
     7681 ctgctgacct cagggctttt cgctttaagg agattaaccc tagtcacagt gattttcatt
     7741 taggagctaa ctaggagttt actctttatg acgtgaaact tcaaagagta tagaaaactt
     7801 ttgtaccaca atcaaagaag aagaaatggt gtatggaaag aaaacaaaac aaaacaagaa
     7861 atctcttgta aaatattcca ggtcaaagtt gtctcctctc caaaccttgc agaagcacct
     7921 ttcttctctt cagcgcactg ttttgggact gtttatgcag cagatgtaag tagacaacat
     7981 ggactccatg tgacatgcct ctaatagtaa agataaagta ttactgaggt taaaaataaa
     8041 aattgagtag tattaattta aagtgcacca tcaggacaac aaaccattta agctgaaaaa
     8101 acgctatttt atttcttgag tttgccagtt gcttccacct tgagttaagg acgtgtctca
     8161 tcttcaccta ctgcgcattc tcccttctct aactgtgtaa tatgtcaggt caaggacatt
     8221 gaatgttatg aattgagaac ctaattgatg cgcatagttt tcatctatgc aattttactt
     8281 gcttctgtca ctttacgatc tgttcatatt tggcatcaat taaagatact ttttaaggat
     8341 cttatcaagg aa
//
