LOCUS       CZK2J00157R_EIF2       11090 bp DNA     linear   UNA 08-NOV-2023
DEFINITION  eukaryotic translation initiation factor 2B subunit epsilon 
            [Source:HGNC Symbol;Acc:HGNC:3261].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000145191
FEATURES             Location/Qualifiers
     source          1..11090
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..11090
                     /label=EIF2B5
                     /note="gene ENSG00000145191
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..10274
                     /label=EIF2B5-210
                     /note="primary transcript ENST00000491144
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 300..10226
                     /label=EIF2B5-229
                     /note="primary transcript ENST00000649814
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 318..1942
                     /label=EIF2B5-205
                     /note="primary transcript ENST00000471832
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 321..10274
                     /label=EIF2B5-226
                     /note="primary transcript ENST00000648915"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 321..10138
                     /label=EIF2B5-220
                     /note="primary transcript ENST00000648314
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 326..10268
                     /label=EIF2B5-203
                     /note="primary transcript ENST00000465218
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 328..1943
                     /label=EIF2B5-201
                     /note="primary transcript ENST00000432569"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 329..10258
                     /label=EIF2B5-215
                     /note="primary transcript ENST00000647636
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 338..10265
                     /label=EIF2B5-225
                     /note="primary transcript ENST00000648890
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 339..10086
                     /label=EIF2B5-223
                     /note="primary transcript ENST00000648682
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 340..9929
                     /label=EIF2B5-216
                     /note="primary transcript ENST00000647909"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 342..10263
                     /label=EIF2B5-228
                     /note="primary transcript ENST00000649688
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 342..10253
                     /label=EIF2B5-221
                     /note="primary transcript ENST00000648599
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 348..10274
                     /label=EIF2B5-207
                     /note="primary transcript ENST00000481054
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 348..10076
                     /label=EIF2B5-224
                     /note="primary transcript ENST00000648882
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(349..543,1575..1699,2583..2768,2861..3038,3129..3209,
                     5043..5120,5381..5693,6888..7033,7200..7341,7465..7566,
                     7742..7849,8015..8105,8405..8528,9062..9187,9560..9670,
                     9847..9906)
                     /codon_start=1
                     /label=EIF2B5-226
                     /note="coding sequence ENSP00000497160"
                     /note="This feature has 16 segments:
                         1:  349 ..  543 / #993366 / ENSE00003835844
                         2: 1575 .. 1699 / #993366 / ENSE00003514319
                         3: 2583 .. 2768 / #993366 / ENSE00003675665
                         4: 2861 .. 3038 / #993366 / ENSE00003643722
                         5: 3129 .. 3209 / #993366 / ENSE00003651593
                         6: 5043 .. 5120 / #993366 / ENSE00003617725
                         7: 5381 .. 5693 / #993366 / ENSE00003492021
                         8: 6888 .. 7033 / #993366 / ENSE00003649614
                         9: 7200 .. 7341 / #993366 / ENSE00003682432
                        10: 7465 .. 7566 / #993366 / ENSE00003536383
                        11: 7742 .. 7849 / #993366 / ENSE00003668116
                        12: 8015 .. 8105 / #993366 / ENSE00003475918
                        13: 8405 .. 8528 / #993366 / ENSE00003534680
                        14: 9062 .. 9187 / #993366 / ENSE00003656619
                        15: 9560 .. 9670 / #993366 / ENSE00003632558
                        16: 9847 .. 9906 / #993366 / ENSE00002454580"
                     /translation="MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQA
                     VLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKE
                     HLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITR
                     ALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLR
                     RFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEE
                     ILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRH
                     NIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQG
                     VRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEE
                     DEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGL
                     KINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVL
                     EINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNY
                     IKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKG
                     QQLRKNQQLQRFIQWLKEAEEESSEDD"
     CDS             join(349..543,1575..1699,2583..2768,2837..3038,3129..3209,
                     5043..5120,5381..5693,6888..7033,7200..7341,7465..7566,
                     7742..7849,8015..8105,8405..8528,9062..9187,9560..9670,
                     9847..9906)
                     /codon_start=1
                     /label=EIF2B5-216
                     /note="coding sequence ENSP00000498164"
                     /note="This feature has 16 segments:
                         1:  349 ..  543 / #993366 / ENSE00003836839
                         2: 1575 .. 1699 / #993366 / ENSE00003514319
                         3: 2583 .. 2768 / #993366 / ENSE00003675665
                         4: 2837 .. 3038 / #993366 / ENSE00001840975
                         5: 3129 .. 3209 / #993366 / ENSE00003651593
                         6: 5043 .. 5120 / #993366 / ENSE00003617725
                         7: 5381 .. 5693 / #993366 / ENSE00003492021
                         8: 6888 .. 7033 / #993366 / ENSE00003649614
                         9: 7200 .. 7341 / #993366 / ENSE00003682432
                        10: 7465 .. 7566 / #993366 / ENSE00003536383
                        11: 7742 .. 7849 / #993366 / ENSE00003668116
                        12: 8015 .. 8105 / #993366 / ENSE00003475918
                        13: 8405 .. 8528 / #993366 / ENSE00003534680
                        14: 9062 .. 9187 / #993366 / ENSE00003656619
                        15: 9560 .. 9670 / #993366 / ENSE00003632558
                        16: 9847 .. 9906 / #993366 / ENSE00003831982"
                     /translation="MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQA
                     VLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKE
                     HLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITR
                     ALEEHSSVPPVLYRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLH
                     FQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFV
                     RGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTT
                     QSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVL
                     DQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVIS
                     LHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEE
                     LQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEEN
                     ISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKA
                     WSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFS
                     QRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD"
     CDS             join(349..543,1575..1703)
                     /codon_start=1
                     /label=EIF2B5-201
                     /note="coding sequence ENSP00000414775"
                     /note="This feature has 2 segments:
                        1:  349 ..  543 / #993366 / ENSE00001741408
                        2: 1575 .. 1703 / #993366 / ENSE00003834077"
                     /translation="MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQA
                     VLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKE
                     HLL"
     prim_transcript 352..10255
                     /label=EIF2B5-211
                     /note="primary transcript ENST00000492226
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 355..10236
                     /label=EIF2B5-222
                     /note="primary transcript ENST00000648630
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 363..7849
                     /label=EIF2B5-202
                     /note="primary transcript ENST00000432982
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 366..11090
                     /label=EIF2B5-204
                     /note="primary transcript ENST00000468748
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 400..10251
                     /label=EIF2B5-219
                     /note="primary transcript ENST00000648256
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 457..2905
                     /label=EIF2B5-230
                     /note="primary transcript ENST00000650244
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 482..10251
                     /label=EIF2B5-231
                     /note="primary transcript ENST00000650270
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 484..10274
                     /label=EIF2B5-209
                     /note="primary transcript ENST00000491008
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 864..2996
                     /label=EIF2B5-214
                     /note="primary transcript ENST00000498831
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     gene            complement(join(1018..11090,1..201))
                     /label=EIF2B5-DT
                     /note="gene ENSG00000272721
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(join(1018..11090,1..201))
                     /label=EIF2B5-DT-201
                     /note="primary transcript ENST00000608135
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(join(1018..11090,1..144))
                     /label=EIF2B5-DT-202
                     /note="primary transcript ENST00000608232
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript 1612..10255
                     /label=EIF2B5-217
                     /note="primary transcript ENST00000648145
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1630..10251
                     /label=EIF2B5-218
                     /note="primary transcript ENST00000648189
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1642..10267
                     /label=EIF2B5-208
                     /note="primary transcript ENST00000484154
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1646..10245
                     /label=EIF2B5-227
                     /note="primary transcript ENST00000649545
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1648..10240
                     /label=EIF2B5-212
                     /note="primary transcript ENST00000492773
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2360..2384
                     /label=PCR Forward
                     /note="color: black; sequence: AGGAAATAACCATGTTTCCTAACCC; 
                     added: 2023-09-29"
     primer_bind     2688..2707
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: TTTGGTGCGCTCTGACTTTC; added:
                     2023-09-29"
     misc_feature    2868..2967
                     /label=Donor Template SNV -> REV
                     /note="color: #00ccff"
     primer_bind     2868..2967
                     /label=Donor Template SNV -> REV
                     /note="color: black; sequence: 
                     CGGAAGCTAGAAAAAAATGTTTCTGTGATGACGATGATCTTCAAGGAGTCATCCCCCAG
                     CCACCCAACTCGTTGCCACGAAGACAATGTGGTAGTGGCTG; added: 
                     2023-09-29"
     misc_feature    2927..2929
                     /label=PAM
                     /note="color: #ffff00"
     misc_feature    2930..2949
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     primer_bind     complement(2930..2949)
                     /label=gRNA Protospacer
                     /note="color: black; sequence: CTTCGTGGCAATGAGTTGGG; added:
                     2023-09-29"
     misc_feature    2938
                     /label=SNV
                     /note="SNV = A
                     REV = G"
                     /note="color: #ff6600"
     primer_bind     complement(3246..3270)
                     /label=PCR Reverse
                     /note="color: black; sequence: CAATAACAGACCCACAGAGTTCTAC; 
                     added: 2023-09-29"
     prim_transcript 5112..5871
                     /label=EIF2B5-213
                     /note="primary transcript ENST00000493740
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 7071..7834
                     /label=EIF2B5-206
                     /note="primary transcript ENST00000479250
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
ORIGIN
        1 ggccccttcg tggcgaccac aagtcctatc acgccacaag aatctcggct tgttcaccta
       61 caaatgaagg ccgacgatac ttgcagaccg agtctgacat ctatgggacc ccactcctac
      121 cctgcgcctc aggggccggg acaaccgtga cccgtaccga ctcctcgcgc ctccgggatc
      181 aacctcagac ccctgtgggc tcccggggaa gacgcatgcg cactgctccc tcctcgtctc
      241 cttcccttcg ttaattagtc catcgcattt caagtgctgg atcctttttg tcccctactg
      301 cgtgcggtgg cagcttcctt gcggaagtgg tgaccgtgag agaagaagat ggcggcccct
      361 gtagtggcgc cgcctggtgt ggtggttagt cgggctaaca agcgcagcgg cgcggggccg
      421 ggaggcagcg gtggcggggg agccagaggg gcggaggagg aaccgccgcc gcccctacaa
      481 gcagttctgg tggccgatag cttcgatcgc cgcttcttcc ccatctccaa ggaccagcct
      541 cgggtgagcg ccgcgcacgc gagcagccag agggcaggaa gggtggcagg gctggagcaa
      601 gttctcattt cggctaacta caacagcatg cccttgaagg acctgcgtct atgctgttat
      661 cctaaaaccg gatgcagagg gactgtctaa accctgatga ctgctgacca agttagtggc
      721 cggcagattt gggcattcgt aatgctgtcc acctcgggtt tgaaaggaat gaagagagct
      781 aaatgtagaa acagcgcttg agagaagcct tcacgcgggg attcagtgcc cctagaagag
      841 gctgcgccgc ttttaacctt gccgatgacg gttgcttagg tgaaaagaaa cgaccttttg
      901 cctctgaaag ggcggtaata tgccttaaaa agagcgggct agatctcagg cagtgtattt
      961 tttttttcct tccaggttct aatgatagcc ggtgctatct gtcgaaagga caaaatgggt
     1021 ttagtggttg attgccatca gcccccgagt tgtctagaca atggggtatt ggactcagct
     1081 gggtcgttca gcagtcagtt cagaaagagg gaactgcatt ggatttaagg aaaatcctct
     1141 ctaattccct cgaaaaagcc taatggctgt tcatttttga caagtgacgg gagatggcta
     1201 acctctgaca cttagaaagg gatcaaggaa ggaaatctta tggggaagaa ggccttagca
     1261 atgcttaaat ttatggaaat atgattgatt tccagtatta tcctttgcca gcagtgaact
     1321 gccattctgt cacagctctg tgtctgatac aaggcacata gattctgtac ttaccatccc
     1381 caaattgcaa tgtctcagac tcaggcttag agcatggcat gaacatcaaa ggcaggaacc
     1441 tgtttatctt tgaattggaa agatacagca aaattacact gtttggaaat acgaatagag
     1501 gagtgaaaat tgttgcagtg gggtacccaa agggattcga gacctcaagt tttttttcat
     1561 cttgtatcct tcaggtcctc ttgcccctgg ccaatgtggc attaattgac tacactctgg
     1621 aattcctgac tgccacaggt gtacaggaaa catttgtctt ttgttgctgg aaagctgctc
     1681 aaatcaaaga acatttactg taaggccctg caacttttct ttccatgttt cgccatcttt
     1741 ttccagtttt ttcaggatga atgtaactaa ggggaaatgt gagaggggaa aaatgtgtct
     1801 acttagatgt cattgtaagt tctaagggta ttttctacac ttatcaggag gtgagcagct
     1861 gaaactttgt atctgtctgt cttgggtttt ctgtgacaaa gattaaagaa taaacttggg
     1921 ttttttctga atctcattat aaagtttggt gccagtggac tttgctttta tttaatggct
     1981 tcagtatgga agttacaatg ataacatctt tgttttccca ctgagtttct aaatcttgtt
     2041 tgttctcacc ttcaaggata taaatgagct ccttgtcatc tttgtattcc cactgcctag
     2101 ctcagtccca agcatatagt gagcacttac atgtttgttg aaatcttgaa aactagggaa
     2161 tcgcattgct tatggaagtc caggatgtaa agactaggac attagcatgg tctccatcag
     2221 acagttccta aatgttatat ggattgcaga aggggaaagc tgacgtgaag cgtgttccca
     2281 ggctactttg tgtaccctag gcttcagcag gattttgagt tcaaattgat gtttagggac
     2341 aaggggtcaa ggtatggaga ggaaataacc atgtttccta acccataaaa caagactggt
     2401 gacacaggat gctgaaagca ttcattatag ttcataatac tctttgaagt tgaagacgct
     2461 ggcaagaacc caaaaaagcc atcgagaagg actgtgagtg ctgaaagggg gtgaaaaatg
     2521 gggaaggctc aaatgatctt tatgcttgat acactcattc ccctcaccct cccttccttt
     2581 aggaagtcaa agtggtgccg ccctacatct ctcaatgtgg ttcgaataat tacatcagag
     2641 ctctatcgat cactgggaga tgtcctccgt gatgttgatg ccaaggcttt ggtgcgctct
     2701 gactttcttc tggtgtatgg ggatgtcatc tcaaacatca atatcaccag agcccttgag
     2761 gaacacaggt caggatggga aaatgacagg aacaagggtt aaagaccagc agagccctga
     2821 gactgctttt ttgcagttct gtccctcctg tcctttatag gttgagacgg aagctagaaa
     2881 aaaatgtttc tgtgatgacg atgatcttca aggagtcatc ccccagccac ccaactcgtt
     2941 gccacgaaga caatgtggta gtggctgtgg atagtaccac aaacagggtt ctccattttc
     3001 agaagaccca gggtctccgg cgttttgcat ttcctctggt gtgtggatat ctggggtcct
     3061 ttgagatggg gaagtgacag gcttcagtgg gagaaacaaa ttaaagtcct tgtaacttct
     3121 ccccacagag cctgtttcag ggcagtagtg atggagtgga ggttcgatat gatttactgg
     3181 attgtcatat cagcatctgt tctcctcagg tgagctcttt agggctgggg ctgcacaccc
     3241 aaagagtaga actctgtggg tctgttattg tccccttaga aggccagggt atatttcttc
     3301 tccctgttta ttttcattat ttttactttt attttttgag acagagtctc gctctgttgc
     3361 ccaggctgga gtgcagtggt gtgatcccag ctcactgcag actctgcctc ctaggttcaa
     3421 gtgattctcc tgcctcagcc tcctgagtag cagggattac aggcacacca ccatgcctgg
     3481 ctaatttttg tatttttagt agagatgggg tttcaccatg ttggccaggc tggtctcgaa
     3541 ctcctggtct caagtgatct acctgtctca gcctcccaaa gtgctgggat tacaggcatg
     3601 agccaccaca cctggcctta ttttcatttt tatttatata tattttttga gacagggtct
     3661 cgctctgtgg cccaggctgg agcacagtgg caccatgact gctcactgca gcctcagcct
     3721 cccaggttca agggaacctc ctgcctcagc ctcctgagta gatggaacca caggcatgca
     3781 ctaccactaa tttttgtatt tttttgtaga gacggggttt tgccatgttg tccggcctgg
     3841 tcacgaactc ctgagctcaa gcaatctgac cacctcagcc ttccaaaatg ctgggattac
     3901 aggcatgagc caccagtttt ttttgttgtt gttgttgtta ttgttgtttt gagatggagc
     3961 ctcgctctgt cgcccaggct ggagtgcagt ggcatgatct cagcttactg cagcctccac
     4021 ctcctgggtt ccagtgattc tcctgcctca gcctctcaag tagctgggac tacaggtacg
     4081 caccaccaca tccggctatt ttttgtattt ttcagtagag atggggtttc accatattgg
     4141 ccaggctggt ctctaactcc tgacctcagg tgatcctccc gccttggcct cccaaagtgc
     4201 tgcaatcaca ggtgtgagcc tctgcaccca gcattttttt tttttttttt tttttttttt
     4261 tgagtcggag tttcactctt gttgcccagg ctggatgcaa tggtgcgatc tcagctcact
     4321 gcaacctcca cctcccaggt tcaagcaatt cccctgcctc agcctcccaa atagctggga
     4381 ttgcaggcat gtgccaccac acccagctaa tttttgtatt tttagtagag acgaggtttt
     4441 accatgttgg tcaggctggt ctcgaactcc caacctcagg tgatctgcct gcctcggcct
     4501 cccaaagtgc tggcattacg ggcatgagcc actgcacctg gccttttttt tttttttaga
     4561 atctattgta aagcaaagat cttgtctcca gtatcccact gggagtctct ttggaattct
     4621 ggtccatgtt cagctgtcat agggtttgct aggtctctgg gggattatct gtgctcctag
     4681 agaatagtac ttaatcctgg ccaagcgtgg tggctcacgc ttgtaatccc agcactttgg
     4741 gaggccgagg tgagtggatc atgaggtcag gagttcaaga ccagcctgac caacacagtg
     4801 aaatcccatc tctactaaaa aataaaaata caaaaattag ctgggcgtgg tggcggacgc
     4861 ttgtaatccc agctacttgg gaggctgagg cacaagaatc actttaaccc aggaggtgga
     4921 ggttgcagtg agccgagatc atgcaactgc actccagcct gggcaacaga gctagacttg
     4981 tctcaaaaaa aaaaaaaaaa gaatagtact taattctagc ccactccact tcccttccac
     5041 aggtggcaca actctttaca gacaactttg actaccaaac tcgagatgac tttgtgcgag
     5101 gtctcttagt gaatgaggag gtgagaaaag tcttccaatg cctctttagg agagaggaga
     5161 aggctatgat tgtatctcat gctggtaact tttgatcttt tttgtatttt attgaggctt
     5221 aggagggagg aaaagcaggg gacaggagga acagtacaca aagaagaatc tttgtggcag
     5281 tgagaggcag aggggaactt aaaaggatta gaaaagttgt acttgggggc attcttcctg
     5341 tctttcttgc ttaggtgacc tccctttcct tctcattcag atcctaggga accagatcca
     5401 catgcacgta acagctaagg aatatggtgc ccgtgtctcc aacctacaca tgtactcagc
     5461 tgtctgtgct gacgtcatcc gccgatgggt ctaccctctc accccagagg cgaacttcac
     5521 tgacagcacc acccagagct gcactcattc ccggcacaac atctaccgag ggcctgaggt
     5581 cagcctgggc catggcagca tcctagagga aaatgtgctc ctgggctctg gcactgtcat
     5641 tggcagcaat tgctttatca ccaacagtgt cattggcccc ggctgccaca ttggtgagca
     5701 caggtgggga atcaagccaa ctatcctagg aacaggaacc tgggggggcc acgtctccag
     5761 aaacacaagg gatggctaca caagggacag tccctgggaa taaggaacta tagagcggct
     5821 acctcaggaa aggaggaaac agggatttga aaacagcgat accttaattt gtagcctgtg
     5881 ggcctctcat tggatgactc ttcacattcc agggtaccct gtagtcacat tcaggagcaa
     5941 gtagataatt aacatgcagt aacaacatct atttagcaca ttacagttta caaagggctt
     6001 ttatatacat tgttacattt aatcttcata atttacaaat aaacaggctc aacaagattt
     6061 tcttgcccaa ggtcattggt aataatggtg ggacctgaat ctgggtattc tgaatgtaag
     6121 tccagtgttc ttttgagtct accataactg cctggttggc ctcttggtat ctcattttgg
     6181 ttgcaggagt gtgatgcgtc taatagtact gcccctttcc aagaacaaca gtaaggctca
     6241 gattctaggg gctgagcgtg gtggctcatg cttgtaatcc tagcacactg ggaagccatg
     6301 gtgggaggat cgcttgagcc caggagttca agaccagcct gggcaacata gcgacacccc
     6361 ttctccacaa atgatacaaa aattagctgg gcatgatggc gcgtgcctgt aatctcagct
     6421 attcgggagg ctgaggcagg aggattacct gagcccagag gtcgagggtg cagtgagtca
     6481 tgatcatact actgcactcc agcctgggtg atggaatgag accgtgtctc aaaagaaaaa
     6541 aaagacttgg tttctaaaat gtaccagaag aggtggaatt atttggttct gcttgtaggc
     6601 gtggatatga ttctaataag gttttttgag ttcagagggt ccccagaaat cctgaaggtt
     6661 ctcgggttct taagctctgt gtgtagtcta taattgttgt aaacttggtg aagtatcttc
     6721 tcaggcctgg gcttaggggg tgagtgggtg tttctggtga cttgcgggat ggggaatgag
     6781 agctaggtgg gagctgtgct ggtgtcagtc agccctgtcc tctatgaagg gctctgctct
     6841 gcggttggaa ccctgagtta cccagaggtc ttcccatcct gagccaggtg ataacgtggt
     6901 gctggaccag acctacctgt ggcagggtgt tcgagtggcg gctggagcac agatccatca
     6961 gtctctgctt tgtgacaatg ctgaggtcaa ggaacgagtg acactgaaac cacgctctgt
     7021 cctcacttcc caggtgagac ctgatctata ctgtgcacag gccctgaatt gcatggcagt
     7081 cacactgagc cagaaggggc attatttcca cccataatcc tgtctaaaaa agttgctctc
     7141 attatcagga tgcacttttc ctccacaccc taatggttct gtgttttttt tccccttagg
     7201 tggtcgtggg cccaaatatc acgctgcctg agggctcggt gatctctttg caccctccag
     7261 atgcagagga agatgaagat gatggcgagt tcagtgatga ttctggggct gaccaagaaa
     7321 aggacaaagt gaagatgaaa ggtgtgagac tcaacaggtg tggggcatct gtgtgtctcg
     7381 ctgcctcata gaagaaccag tgtttcctcc tggagggatt ggtgcttccg ccgggccctc
     7441 tctatagatc attgcctttt ccaggttaca atccagcaga agtaggagct gctggcaagg
     7501 gctacctctg gaaagctgca ggcatgaaca tggaggaaga ggaggaactg cagcagaatc
     7561 tgtggggtga gctaggcctg atgcctgccc ttctcctcct gaatcggaat attttgaagg
     7621 ataatgaata cttcagagtc acattactta ttcactcctt tattcaggca gacagggcag
     7681 gtatattgct ctctgtcaat gactcttttt ttctttttcc tcacccatta tggcttctca
     7741 ggactcaaga tcaacatgga agaagagagt gaaagtgaaa gtgagcaaag tatggattct
     7801 gaggagccgg acagccgggg aggctcccct cagatggatg acatcaaagg tgagtggcag
     7861 gggagaaatg cgctggacca gtttattctc tgcctggatc aactagccag aggcttacgt
     7921 tcctcagaaa gggtttggta tcgagtcaag actagatgac ttagagcatt ctgaatgatg
     7981 ttggcccatg aacttatcct tgctttgatt tcagtgttcc agaatgaagt tttaggaaca
     8041 ctacagcggg gcaaagagga gaacatttct tgtgacaatc tcgtcctgga aatcaactct
     8101 ctcaagtaag agcagcccct ccctgttctc ctcggggtga tcccgggaag gtagaggctt
     8161 tctcgtaagt gttttgtctc caaataggaa cctattcctt cgacatccca aatggaaaga
     8221 ccagtagtat ttggagcaag gagagcatta ttaagttcta gcctcagcat agactttctc
     8281 cttcctaaac cctccccttc catattgttc catccagatt cctctccaat gtctatcaaa
     8341 gtcatagttc taagcctgct gaaaggccag tgaaggccct ggtgtcaccc cagtctcccc
     8401 acaggtatgc ctataacata agtctaaagg aggtgatgca ggtactgagc cacgtggtcc
     8461 tggagttccc cctgcaacag atggattccc cgcttgactc aagccgctac tgtgccctgc
     8521 tgcttcctgt gagcaaagat tggagctgag tacaagggat tgggtacagg caaaggaaat
     8581 caggagtaga ctgtcttgtt atattgggtg tatgtcactt ctggttcctt tttccttgcc
     8641 tctggaagct ggttgggctc tagagcagcc ttggctggtt cagaggggtc agattgagtt
     8701 caatactgac tggcctttga gactccagga tttcatacca tcctaggccc acaaaggtat
     8761 attcagaatg gacctggaca ttcagactct atggcagcaa tttgacccaa cacaagatca
     8821 gcctctgggg tgggaatagg tattttgagg cagcctatag catctgatcc cctttcacct
     8881 tccctaagga atctcttttt cccaaaccct gtcaacaact cccaagtccc tctgacataa
     8941 ttccacagaa cctgtatcat ctcccttttg tccacccatg tcatcagtat ggatttgctt
     9001 ggatgtccag gtataggtga atgcttagga atatactgca gctcccttct ttcttccata
     9061 gctgctaaag gcctggagcc ctgtttttag gaactacata aagcgcgcag ccgaccattt
     9121 ggaagcgtta gcagccattg aggacttctt cctagagcat gaagctcttg gtatttccat
     9181 ggccaaggtg aatatgacct caagccccat tcttctgcac ttgctttcaa acgagggttg
     9241 gtgaccccct tgggagacat aaaatagaag ctaatgtgaa tccagtatgg accatccact
     9301 cccaatatgc ttattgctag aatagtacag cttggagccg gactaataag gtagccatct
     9361 ggggggtggt ctggaaatgt gcaaagctct ccccagcaat agttccagaa gttagacctg
     9421 ccccaggagc agagcgaaca gctcactctt gtccgttttg gaggaggcta ctcagtgcat
     9481 agagggatct gagggcctgg tagagtatcc tgctggactt tccaaggccc ctgaggtccc
     9541 ctttattggc cttttgcagg tactgatggc tttctaccag ctggagatcc tggctgagga
     9601 aacaattctg agctggttca gccaaagaga tacaactgac aagggccagc agttgcgcaa
     9661 gaatcaacag gtgcgtcagg ctgtcctcct ctcgccaaga tggttatctc attcccaggt
     9721 cctggcccct agactctagg gtggttggtt tctggggctc tggttgttag agctgtttat
     9781 ctgccgaggg gagcaggaga gttatgtgca cctcccccag ccgatctctc ctctgcttct
     9841 ttacagctgc agaggttcat ccagtggcta aaagaggcag aagaggagtc atctgaagat
     9901 gactgaagtc acactgcctg ctcctttggg tgtgattgag tgccctcctg gctcctgggc
     9961 tgggacaagt gaggaactag ctgcagaggg atgagtgacc accatccagg ctgagactga
    10021 aaggagcaga ggctggaact acagtattct ttcccctgct agcaaccatg tgcctcccat
    10081 cctgactgtg gagttgggat gtggaagtgg ggctggaaca aagcttctgc ctagggagga
    10141 gctaagcagg cccggcagtt ggaggaaggc cagaggaaca gctttgtgct ccggctttcc
    10201 ctcagggaac agcagagagc agttggctct ttctgctgct tgtatatgtt aatattaaaa
    10261 gagagagtgg tgtatttggt ttgtctccat ccctgactaa tcagccagtg aagtatgtga
    10321 ccagaatcac atgatagcct ttccttaaca cctgggggag agggaggacg ggtgtgccag
    10381 ccactaggtg gtactgtggt accttgctaa ttaacctttc ccatggctca tgggtagtgg
    10441 agggcggagg ttcttttcca gcctctgtgg accaacttgt gatcctgggg tggtgtgcct
    10501 aagcccgttt ccatagagtc ccattttgaa cacttttctg ccagaggatt atgttgccaa
    10561 ccatatgtaa agcccattag acatgccagt tcctttcaca tgccttttcc agttgatctg
    10621 gtactcgttc tgtcaggaag ctgggccacg cttcatcaga aataaggaaa tcagctcaag
    10681 gtcacatgac tcttgagggg taaagctaag gacttgaccc tcagaatcca gaactctgtc
    10741 cttcagcgta ttgtgtttca gtgaagggaa aattggcccc acagacaaat ataacccaga
    10801 gccagtaatg gaggtcccaa gaacacagga aggccatgtg aagggtgctt tcccttgaga
    10861 gtgtttacta tgtgacaggc aagcagtttt cacatattgt ttcatttaat cctcacatga
    10921 gccccatgaa gtcttatccc cattttcaga taaggtgcct gaagctcaca gagcttccct
    10981 agctagtaac tgacagagcc aggtgtgata ttggactctt gacctggagc ttgttacttt
    11041 gctgtattgc tttaacctta accaaaagag acatgccttt ctgtcttttc
//
