LOCUS       CZK2J00159_SOD1_        9766 bp DNA     linear   UNA 13-OCT-2023
DEFINITION  superoxide dismutase 1 [Source:HGNC Symbol;Acc:HGNC:11179].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2024 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000142168
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     misc_feature    1..500
                     /label=Partial Promoter
                     /note="color: #cc99ff"
     primer_bind     293..317
                     /label=PCR Forward
                     /note="color: black; sequence: agaaggttgttttctccacatttcg; 
                     added: 2023-10-06"
     primer_bind     443..461
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: gtgcgaggcgattggtttg; added: 
                     2023-10-13"
     gene            501..9766
                     /label=SOD1
                     /note="gene ENSG00000142168
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 501..9766
                     /label=SOD1-202
                     /note="primary transcript ENST00000389995"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 528..9766
                     /label=SOD1-201
                     /note="primary transcript ENST00000270142"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 528..8176
                     /label=SOD1-204
                     /note="primary transcript ENST00000476106
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 544..9766
                     /label=SOD1-203
                     /note="primary transcript ENST00000470944
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    595..694
                     /label=Donor Template WT -> SNV
                     /note="color: #00ccff"
     primer_bind     complement(595..694)
                     /label=Donor Template WT -> SNV
                     /note="color: black; sequence: 
                     ctccgtcccagcccttgcCTTCTGCTCGAAATTGATGATGCCCTGCACTGGGCCGTCGC
                     CCTTCAGCACGCACACGACCTTCGTCGCCATAACTCGCTAG; added: 
                     2023-10-05"
     CDS             join(605..619,4625..4721,7284..7353,8093..8210,9306..9413)
                     /codon_start=1
                     /label=SOD1-202
                     /note="coding sequence ENSP00000374645"
                     /note="This feature has 5 segments:
                        1:  605 ..  619 / #993366 / ENSE00001723323
                        2: 4625 .. 4721 / #993366 / ENSE00003492976
                        3: 7284 .. 7353 / #993366 / ENSE00003624439
                        4: 8093 .. 8210 / #993366 / ENSE00003555033
                        5: 9306 .. 9413 / #993366 / ENSE00003902052"
                     /translation="MATKAESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPH
                     FNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEK
                     ADDLGKGGNEESTKTGNAGSRLACGVIGIAQ"
     CDS             join(605..676,4625..4721,7284..7353,8093..8210,9306..9413)
                     /codon_start=1
                     /label=SOD1-201
                     /note="coding sequence ENSP00000270142"
                     /note="This feature has 5 segments:
                        1:  605 ..  676 / #993366 / ENSE00001507447
                        2: 4625 .. 4721 / #993366 / ENSE00003492976
                        3: 7284 .. 7353 / #993366 / ENSE00003624439
                        4: 8093 .. 8210 / #993366 / ENSE00003555033
                        5: 9306 .. 9413 / #993366 / ENSE00003902052"
                     /translation="MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHG
                     FHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSV
                     ISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ"
     misc_feature    613..632
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     primer_bind     613..632
                     /label=gRNA Protospacer
                     /note="color: black; sequence: GAAGGCCGTGTGCGTGCTGA; added:
                     2023-10-05"
     misc_feature    618
                     /label=SNV
                     /note="WT = C
                     SNV = T"
                     /note="color: #ff6600"
     misc_feature    633..635
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(1314..1338)
                     /label=PCR Reverse
                     /note="color: black; sequence: agcccaatcatgacctagaacagag; 
                     added: 2023-10-13"
     gene            5141..5317
                     /note="gene ENSG00000238390
                     snoRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 5141..5317
                     /note="primary transcript ENST00000458922
                     snoRNA"
                     /note="color: #a6acb3; direction: RIGHT"
     gene            complement(7563..8082)
                     /note="gene ENSG00000273271
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(7563..8082)
                     /note="primary transcript ENST00000609934
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 atactaagga ctaagaaaat tgcaggggaa gaaaaggtaa gtcccgggat tgaggtgtag
       61 cgactttcta taccctcaga aaactaaaaa acaagacaaa aaaatgaaaa ctacaaaagc
      121 atccatcttg gggcgtccca attgctgagt aacaaatgag acgctgtggc caaactcagt
      181 cataactaat gacatttcta gacaaagtga cttcagattt tcaaagcgta ccctgtttac
      241 atcattttgc caatttcgcg tactgcaacc ggcgggccac gcccccgtga aaagaaggtt
      301 gttttctcca catttcgggg ttctggacgt ttcccggctg cggggcgggg ggagtctccg
      361 gcgcacgcgg ccccttggcc ccgcccccag tcattcccgg ccactcgcga cccgaggctg
      421 ccgcaggggg cgggctgagc gcgtgcgagg cgattggttt ggggccagag tgggcgaggc
      481 gcggaggtct ggcctataaa gtagtcgcgg agacggggtg ctggtttgcg tcgtagtctc
      541 ctgcagcgtc tggggtttcc gttgcagtcc tcggaaccag gacctcggcg tggcctagcg
      601 agttatggcg acgaaggccg tgtgcgtgct gaagggcgac ggcccagtgc agggcatcat
      661 caatttcgag cagaaggcaa gggctgggac ggaggcttgt ttgcgaggcc gctcccaccc
      721 gctcgtcccc ccgcgcacct ttgctaggag cgggtcgccc gccaggcctc ggggccgccc
      781 tggtccagcg cccggtcccg gcccgtgccg cccggtcggt gccttcgccc ccagcggtgc
      841 ggtgcccaag tgctgagtca ccgggcgggc ccgggcgcgg ggcgtgggac cgaggccgcc
      901 gcggggctgg gcctgcgcgt ggcgggagcg cggggaggga ttgccgcggg ccggggaggg
      961 gcgggggcgg gcgtgctgcc ctctgtggtc cttgggccgc cgccgcgggt ctgtcgtggt
     1021 gcctggagcg gctgtgctcg tcccttgctt ggccgtgttc tcgttcctga gggtcccgcg
     1081 gacaccgagt ggcgcagtgc caggcccagc ccggggatgg cgactgcgcc tgggcccgcc
     1141 tggtgtcttc gcatccctct ccgctttccg gcttcagcgc tctaggtcag ggagtcttcg
     1201 cttttgtaca gctctaaggc taggaatggt ttttatattt ttaaaaggct ttggaaaaca
     1261 aaaatacgca acagagaccg tttgtgtgac actttgcagg gaagtttgct ggcctctgtt
     1321 ctaggtcatg attgggctgc aagggcagag aaggtagcct tgaacagagg tccttttcct
     1381 cctcctaagc tccgggagcc agaggtttaa ctgacccttt tggggatttt tgagggcagt
     1441 gatcttaact ttgggtgcac agttagctta tttgaagatc ttactaaaaa tacaccagag
     1501 cccaacctcc gaccaattac atcaaaacct gtcctagtgc agggtgagta ttgctgtttt
     1561 ttgaaagttt ccaaaagtga ttttgatgtg cacctacgat tgagaactgt cgtttgagga
     1621 cagtgggtgg agtttcgtat ttggaaatta gaagacctgg agtttccatt acaccgaatt
     1681 ggcacttaat aactgttgtc ggagcatttc ttaagccaca ttttcgtaaa gtggctttaa
     1741 aattgctctg ccagtaggca ggttgctaag atggtcagag acaaacttct gaacgactct
     1801 tgtaaaatat acagaaatat tttcagaact tttatcagta aaattacaaa acgtgttgca
     1861 aggaaggtgc ttgtgataac actgtcccca gaaccttagt gaagttacca actggtggaa
     1921 aattttctct tgcactcggc ttaaaaatca tgagggaata tttactatac gaatgagatt
     1981 cagtctttaa aggggtttac agaaacgtga gaggacagga acagttagtc tgtgtaaatg
     2041 tctgaaatat atgtgaggga gataatgagt ttagcctttt tctttaatag gtctccagat
     2101 tttctggaaa aggttctttg gcatttgact ccattttgct gtttcatttg tcagacttct
     2161 ttttgtccct ctttacttct ccccacataa ttcaccagta ctagtgtttt gtttttcaga
     2221 ccaagtctcg ctctgtcgcc caggctggag tgcagtggcg cgatctcagc tcactgcaac
     2281 ctccgcctcc caggttcaag caattctcct gcctcagcct cccgggtagc tgggactaca
     2341 ggcgcgcgcc gccacgcctg gctaattttt tatattttag tagagacggc gtttcaccat
     2401 gttggccagg atggtctcga tctgttgacg tcgtgatcca cccgcctcgg cctcccaaag
     2461 tgctgggatt acaggcgtga gccaccccgc ccggccacca gtgctattct taagacgcct
     2521 ctgaggaatc ccttctccct ggccattgag aatccatgca tgaacccagg ttttccacct
     2581 tccctgagca gcttgcatag ttccttcttt taagcgcctg acttcgtttt gtttggtgcc
     2641 cgttgtacct gagaatgagc cttggatagt ggagcattcc agctttccag atatgcagag
     2701 ataatacatt ggctatcagc tacttggctt ggcctattcc gtgtttaaaa tcttggactc
     2761 tttgctagtt tttacagatc agaatttttc acgtattaat ccagttttcc tagcttctct
     2821 tgaagaattt ttggagatct cttcatactg agccttcatt agcccaggac agtactgctg
     2881 tagcagttca tatatttttt cgcttcccag gcctgtgtta ttcacttaag ttcatagcct
     2941 ggtccctgca gggttgtacc cgagcacagc tacttagatg tcctgaatgt attaccggtt
     3001 aaatggaggt ttcaaagaac ctgctgtttt tggccctgtg ctcttgataa cagagtgttt
     3061 gagggacaac tttcacattt gagtttttcc aaaattaaag gttgtagaag agtcacagta
     3121 tctattgtca aaaagaaaag aatttaaaaa ggcagcaatt gccaggatac ttcatttgag
     3181 caatgatatt ttccagtgga aagtcacatc ttaagggtta atgcccctta actgttggcc
     3241 gtatttgaaa acaaaccaag ctaaaaacaa gagacactga catgttgtat gacggtgtgg
     3301 tgtggatgtt gtgtttattt tagtcctgag atctagttgt aacttccttg atttctgtat
     3361 gtagccacgg agcaccatta cctgtcacca ttacctgaat ggctatactg cttgctttca
     3421 ttttggtaga gtggaaaggt tacctaggtt tcagtgcttg aaaagatttc agaaagcagt
     3481 agtacgtctg gttagactag aatcagtcct ctcctggggg cagtggaata taatattttc
     3541 tgactgctaa ttaaaaatac ctgtgatagc cgggcgtggt ggcttacgcc tgtaatccca
     3601 gcactttggg aggccgagac gggtggatca cgaggtcagc agatggagac catcctggct
     3661 aacacggtga aaccccgtct ctactaaaaa tgcaaaaaaa ttagccgggt gtggtggtgg
     3721 gcgcctgtag tcccagctac tcaggaggct gaggcaggag aatggcatga acctgggagg
     3781 cggagcttgc agtgagccga gatcatgtca ctgcactcca gcctgggcga cagagcgaga
     3841 ctcgtctcaa aaaaaaaaag aaaaaaactt atgatggaca cttaaaaaca ctcactgagt
     3901 ggggagtgga gagcaggggt cccagggtag cctgttggac atttccaggg cgactttttc
     3961 tttttttttt tttaaagtca agtgagtatg ccatatggaa aagggtgtgc gtggagaaaa
     4021 agcaaggggc tccagagtgt aggatgagac atacaccttt tgggttaaaa aggctgaggc
     4081 aggagaatgg cgtgaacccg ggaggcggag cttgcagtga gctgagatca tgccactgca
     4141 ctccagcctg ggcgacagag cgagactctt gtctcaaaat aaaaaacgtt tacatgtaca
     4201 tgtatattca acatgtacaa atataaccta ttcaaaagta tttactacat aaataggtac
     4261 ttacattacc tatttactgt aatagtcaaa gcctatgaag tatctaacac tgatgtgtag
     4321 gtactcactt tgcttgccac tctattaggt gctttttatg ttatttaatc atgaagcctg
     4381 gccacagggt gcttgtgcat tgagtgtggg aacaagatta ccatctccct tttgaggaca
     4441 caggcctaga gcagttaagc agcttgctgg aggttcactg gctagaaagt ggtcagcctg
     4501 ggatttggac acagattttt ccactcccaa gtctggctgc tttttacttc actgtgaggg
     4561 gtaaaggtaa atcagctgtt ttctttgttc agaaactctc tccaactttg cacttttctt
     4621 aaaggaaagt aatggaccag tgaaggtgtg gggaagcatt aaaggactga ctgaaggcct
     4681 gcatggattc catgttcatg agtttggaga taatacagca ggtgggtgtt gtgctgtgct
     4741 ggtgacccat acttgttcac cctagttaga taaacagtag agtagcccct aaacgttaaa
     4801 acccctcaac ttgtttttgt ttttgagaaa gggtcttgct ctgtcgctca ggctggagtg
     4861 cagtggcgct gtgcgatcat ggctgacctt agccttgacc tcccaggctc cattgatcct
     4921 catgccttgg cccgtagctg ggactacagg tacacaccac cacgcctggc taatttttgt
     4981 atttttttct agaggtgggg tttcatcatg ttgcccaggc tggtcttgaa ctgctgggct
     5041 caagtggtct atcctcctcg acctcccaaa gtgctgggat tacatgtgtg agccactgtg
     5101 cctgggaaaa ccctcaactt ttcttttaaa aaagaggtca actttattgt atataagcac
     5161 tgtgctaaaa ttgcaggaac tgggaccata tcctgatttt tgtaataatg ccagcagagt
     5221 acacacaaga aaagtaactg cactagattg tgaagactgg ggtggacctg cttctgaagg
     5281 tccagtgccc tttgtcttaa gatttggtgt agtgtgtctt tagaaaccaa aaaaagagaa
     5341 gaagatcaac cttaagatta gccacaaaac tgggctttga tacctaggtg tggaaaagaa
     5401 agggaaagag ttgatgtttt gtcttacagc atcattgtag aagagggtgt ttttttgttt
     5461 gtttgttttt tgagacggag tcttactctg tggcccaggc tggagtgcag tggcgcgatc
     5521 tcggctcact gcaagctccg cctcccgggt tcatgccatt ctcctgcctc agccccctga
     5581 gtagctggga ctacaggtgc ccgccacccc gcctggctaa ttttttgtat ttttagtaga
     5641 gacggggttt cactgtgtta gccaagatgg tctctctcct gacctcgtga tccgcctgtc
     5701 tcagcctccc aaagtgctgg gattacaggc atgagccacc gcacccagcc agaagagggt
     5761 gttttttaaa gaaggcaaat aggaaataaa aacttgggct cttaactttt gtaatgatcc
     5821 caggtgtttg agctgggggt tgagggtggg tgcctcgagc aaaggggctg catttatttg
     5881 cataatgcca tgtaagagta gctctacacc ccaaacacag gcttcttagt gggaccaaag
     5941 tatgatacaa actgaagatg gaatgcagag gattattggt actttggaat atgcttaaaa
     6001 aaaatttttt taaagtattt ttaaaaaatc aggcaacccc tgaaccagag taggttcaga
     6061 gaaactgcca aattttattt tcttaatttg ggattggaag caagttaaca gaagtttatg
     6121 agttaagttg catttagtga tcttttgcca tatttgagta ataatctgat ttttttgttt
     6181 atagatttct tcttaaatta actttattca tcttgctaat ttagtttcaa atagtgattt
     6241 gtaatgatca gatttgatcc atttctgtaa ttgctgaaat tcccccgagt tgctttttgg
     6301 ctttaccgcc tctggtctgg gaggtgattg ctctgctgct tcctgtaact tgcctgcctt
     6361 tctccctgtg tgggactcct gcgggtgaga gcgtggctga agacagccgt gttatgaaag
     6421 ggcctcctgt gctgtcgagg ttgtgctctg tgaatgtcat cccctggtgc acagcagcac
     6481 cttctacaca ggatacagtt ggaatgccgc cccctcgagt tgtgtaaggc agcagccttg
     6541 gcccttgcac ataagatgct gttgaatatt ctgcctgcac caagtaaagg gcacagatag
     6601 aactgcttgg catatgttgc tggggagatg agttttttgt aaagtatact acgttcttaa
     6661 gaatttggat cataaccatg ggattttaat aatagaaaaa ctgttgaaga tcagtctggt
     6721 cccttatttt tacagtgaag aagccaaagc ccagagaagg gtgttaactt tacaagtgtc
     6781 agacagtagt tagaacttgg tggggttttt tttttttttt ttttgagatg gagtcttgct
     6841 ctgttgccca ggctggagtg cagtggtgcg atctcagctc actgcaacct ctgcctccca
     6901 ggttcaagcg attctcctgc ctcagcctac taagtagctg ggactatagg tgcgcaccac
     6961 cacgcctagc taatttttgt attttttcag tagagacagg gttttgctat gctggccagg
     7021 ctggtctcaa actcctgacc tcagatgatc cagccacctc agcttcccaa agtgctgggg
     7081 ttccaggtgt tagccaccat gcctggccat agacttgttt ctgttccctt ctcactgtgg
     7141 ctgtaccaag gtgttgctta tcccagaagt cgtgatgcag gtcagcactt tctccatggg
     7201 aagttttagc agtgtttctt tttagaatgt atttgggaac tttaattcat aatttagctt
     7261 ttttttcttc ttcttataaa taggctgtac cagtgcaggt cctcacttta atcctctatc
     7321 cagaaaacac ggtgggccaa aggatgaaga gaggtaacaa gatgcttaac tcttgtaata
     7381 atggcgatag ctttctggag ttcatatggt atactacttg taaatatgtg ctaagataat
     7441 tccgtgtttc ccccaccttt gcttttgaac ttgctgactc atctaaaccc ctgctcccaa
     7501 atgctggaat gcttttactt cctgggctta aaggaattga caaatgggga cacttaaaac
     7561 gatttggttt tgtagcattt attgaatata gaactaatac aagtgccaaa ggggaactaa
     7621 tacaggaaat gtcatgaaca gtactgtcaa ccactagcaa aatcaatcat cattgtgaaa
     7681 cataggaagc ttctgtagat aaaaaaaaaa attgatactg aaaactagtc gagactccat
     7741 ttatatgtgt atgttttctg aaagcctttc agaaaaatat taaatttaag gacaagattt
     7801 ttatatcaga ggccttggga catagctttg ttagctatgc cagtaattaa caggcataac
     7861 tcagtaactg agagtttacc ctttggtact tctgaaatca ggtgcagccc catctttctt
     7921 cccagagcat tagtgtgtag acgtgaagcc ttgtttgaag agctgtattt agaatgccta
     7981 gctacttgtt tgcaaatttg tgtctactca gtcaagtttt aatttagctc atgaactacc
     8041 ttgatgttta gtggcatcag ccctaatcca tctgatgctt tttcattatt aggcatgttg
     8101 gagacttggg caatgtgact gctgacaaag atggtgtggc cgatgtgtct attgaagatt
     8161 ctgtgatctc actctcagga gaccattgca tcattggccg cacactggtg gtaagttttc
     8221 ataaaaggat atgcataaaa cttcttctaa catacagtca tgtatctttt cactttgatt
     8281 gttagtcgcg gtttctaaag atccagataa actgtacttg cagttcaaat taggaaaagc
     8341 aattttattg gacaattacg gtgaaaatga attattttat ctaggtcagt taagaacact
     8401 gttctgctaa gatgcagtaa aaagcaggtt acatttgacc atattagatc tgagtttgga
     8461 aaacagaagt agtctttagt tttaaaatgg ccagattttc ttgccaggat tgggtttctc
     8521 acttgttaaa cagaacattt tgttaagttt aaaacctggg atggacttaa gtattcatgt
     8581 tcattcatgt tcattcagga ctgcaggtta tcatgacttg tttaacttgt gggaagctgt
     8641 tgtcccaagt tatcctgggg aactgcatct ggttcttgca aaacaccaag tagacaggct
     8701 ctcttttacc tccccttgag ggcattaaca ttcagtagtc acttccattc agttaaccct
     8761 ttatttttat ggtttttctt gagccatagt tgtaaagcag aaaaatcatt tataaaggtt
     8821 tgttgaacaa aattcaaaat actgttgctt aaagtattaa gattttttag gattatacct
     8881 tacttatagg cccgtcattc atttggcatg aaattttgag ttttattcac tttcactttc
     8941 ctttttttcc aaagcaatta aaaaaactgc caaagtaaga gtgactgcgg aactaaggtt
     9001 actgtaactt accatggagg attaagggta gcgtgtggtg gtctacaaca tagttatttg
     9061 ggttttagta tttcatttag acagcaacac ttacctaatg tttaaaggta atgtctttgc
     9121 aacaccaaga aaaagctttg agtagtagtt tctactttta aactactaaa tattagtata
     9181 tctctctact aggattaatg ttatttttct aatattatga ggttcttaaa catcttttgg
     9241 gtattgttgg gaggaggtag tgattacttg acagcccaaa gttatcttct taaaattttt
     9301 tacaggtcca tgaaaaagca gatgacttgg gcaaaggtgg aaatgaagaa agtacaaaga
     9361 caggaaacgc tggaagtcgt ttggcttgtg gtgtaattgg gatcgcccaa taaacattcc
     9421 cttggatgta gtctgaggcc ccttaactca tctgttatcc tgctagctgt agaaatgtat
     9481 cctgataaac attaaacact gtaatcttaa aagtgtaatt gtgtgacttt ttcagagttg
     9541 ctttaaagta cctgtagtga gaaactgatt tatgatcact tggaagattt gtatagtttt
     9601 ataaaactca gttaaaatgt ctgtttcaat gacctgtatt ttgccagact taaatcacag
     9661 atgggtatta aacttgtcag aatttctttg tcattcaagc ctgtgaataa aaaccctgta
     9721 tggcacttat tatgaggcta ttaaaagaat ccaaattcaa actaaa
//
