Protocol 17584: Standard PCR Assay - Laragen

NOD.Cg-Rag1^tm1Mom Il2rg^tm1Wjl/SzJ

Stock No: 007799

Version 2.0

Notes

27410 expected CAG size is between 180 and 200

For additional information please contact Laragen directly:

Jin Li

Laragen, Inc.

10601 Virginia Ave. 

Culver City,  CA 90232

310 280 0804

jin@laragen.com

www.laragen.com

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The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note

Strains Using This Protocol

Stock Number	Strain Name
037010	129;B6-Fmod^tm1Aol/SooJ
007664	129S-Efnb1^tm1Sor/J
035806	129S4/SvJae-Fgfr2^tm6.1Sor/Mmjax
037318	B6(Cg)-H3c6^em1Sjes/J
037951	B6.129(Cg)-Wapl^tm1.1Kpfe/J
003558	B6.129-Grm5^tm1Rod/J
037071	B6.129-Gt(ROSA)26Sor^tm1(CAG-cas9*,-EGFP)Fezh Is(14)2Rdf/CjwuJ
039728	B6.129-Gt(ROSA)26Sor^tm1(CAG-Sall4_ia,-Sall4_ib,-EGFP)Ykawa/J
037698	B6.129-Gt(ROSA)26Sor^tm13.1(CAG-HRP)Luo/J
030952	B6.129-Gt(ROSA)26Sor^tm5.1(CAG-Sun1/sfGFP)Nat/MmbeJ
038594	B6.129-H3f3a^tm1Hjf/J
003598	B6.129-Htt^tm5Mem/J
034643	B6.129-Irgm1^tm1Gat/J
037206	B6.129-Optn^tm1.1Htse/J
003870	B6.129-Plin1^tm1Chan/J
034226	B6.129-Rab25^tm1Jrgo/J
036108	B6.129P2(C)-Cnr1^tm1.1Ltz/J
004595	B6.129P2-Htt^tm2Detl/150J
021193	B6.129P2-Htt^tm2Detl/365J
034729	B6.129P2-Mylip^tm1Pton/J
037673	B6.129S-Atxn1^em3Hzo/J
034595	B6.129S-Capn1^tm1Ahc/J
036668	B6.129S-Grpr^tm2(icre)Zfc/J
038900	B6.129S-Mylk^tm2Avs/J
027411	B6.129S1(Cg)-Htt^tm2Mem/20ChdiJ
007015	B6.129S1-Gadd45g^tm1Flv/J
037386	B6.129S1-Gnat3^tm1Rfm/J
036156	B6.129S1-Htt^em2(HTT)Chdi/J
029928	B6.129S1-Htt^tm1.1Mfc/190ChdiJ
036157	B6.129S1-Htt^tm8(HTT*)Szi Htt^em3(HTT)Chdi/J
037918	B6.129S1-Htt^tm8(HTT*)Szi/Mmjax
035475	B6.129S1-Ifi30^tm1Cres/J
038977	B6.129S1-Nfkb2^tm2Brv/AlexJ
027412	B6.129S4-Htt^tm1.1Pfs/80ChdiJ
004185	B6.129S4-Soat2^tm1Far/J
037536	B6.129S4-Sypl2^tm1Hta/J
010672	B6.129S4-Tnfrsf11b^tm1Eac/J
039415	B6.129S6(Cg)-Asah1^tm1Medin/J
003742	B6.129S6-Cdh6^tm1Sma/J
039210	B6.129S6-Tnfaip8l2^tm1Yhcn/J
007218	B6.129S6-Trp53^tm2Xu/J
005601	B6.129S7-Atxn1^tm1Hzo/J
002616	B6.129S7-Hba^tm1Paz/J
002277	B6.129S7-Src^tm1Sor/J
038214	B6.C(Cg)-Gt(ROSA)26Sor^tm1(Stat6*)Voeh/J
036101	B6.Cg-Atxn1^em2Hzo/J
038957	B6.Cg-Cartpt^tm1(cre)Hze/J
035514	B6.Cg-Cd19^tm1(cre)Cgn Ikzf3^tm1.1Cjwu/J
039105	B6.Cg-Ces1g^tm1Reli/J
012329	B6.Cg-Fxn^tm1.1Pand Fxn^tm1Mkn/J
012910	B6.Cg-Fxn^tm1Mkn Tg(FXN)YG22Pook/J
012253	B6.Cg-Fxn^tm1Mkn Tg(FXN)YG8Pook/J
038190	B6.Cg-Gt(ROSA)26Sor^tm1(CAG-Bcl2)Zhu/J
031279	B6.Cg-Gt(ROSA)26Sor^tm1(tetO-Yap1*,-GFP*)Stang/J
027430	B6.Cg-Gt(ROSA)26Sor^tm47(HTT)Arte/ChdiJ
027429	B6.Cg-Gt(ROSA)26Sor^tm48(HTT)Arte/ChdiJ
027428	B6.Cg-Gt(ROSA)26Sor^tm49(HTT)Arte/ChdiJ
027427	B6.Cg-Gt(ROSA)26Sor^tm50(HTT)Arte/ChdiJ
027426	B6.Cg-Gt(ROSA)26Sor^tm51(HTT)Arte/ChdiJ
027431	B6.Cg-Gt(ROSA)26Sor^tm52(HTT)Arte/ChdiJ
027435	B6.Cg-Htt^tm1(HTT)Arte/ChdiJ
036155	B6.Cg-Htt^tm1(HTT*)Chdi Htt^em1(HTT)Chdi/J
034191	B6.Cg-Mir21a^tm1Yoli/RthsnJ
007001	B6.Cg-Ndor1^Tg(UBC-cre/ERT2)1Ejb/1J
008085	B6.Cg-Ndor1^Tg(UBC-cre/ERT2)1Ejb/2J
038955	B6.Cg-Npr3^tm1(cre)Hze/J
029721	B6.Cg-Pvalb^tm1(cre)Arbr Fxn^em2Lutzy Fxn^em2.1Lutzy/J
038958	B6.Cg-Rorb^tm1(cre)Hze/J
036415	B6.Cg-Sh2b1^em3Ccsu/J
038959	B6.Cg-Slc17a8^tm1(cre)Hze/J
034601	B6.Cg-Tgfb1^tm1Mki/J
038954	B6.Cg-Vipr2^em1(cre)Hze/J
029436	B6.Cg-Tg(CMV-AR*113Q)FMonk/J
008586	B6.Cg-Tg(FXN*)1Sars Fxn^tm1Mkn/J
006471	B6.Cg-Tg(HDexon1)61Gpb/J
033705	B6.Cg-Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik/J
035020	B6.D1-Ksr2^tm1Rlw/J
036551	B6.FVB(Cg)-Tg(Pmch-cre)1Lowl/J
027432	B6.FVB-Tg(YAC128)53Hay/ChdiJ
033109	B6;129-Cdh2^tm1(cre/ERT)Linli/J
038489	B6;129-Ces1d^tm2Reli/J
039578	B6;129-Eya1^tm1Px/J
039579	B6;129-Eya1^tm3(cre/ERT2)Px/J
039577	B6;129-Eya2^tm2Px/J
036087	B6;129-Gt(ROSA)26Sor^tm3Cos/J
021039	B6;129-Gt(ROSA)26Sor^tm5(CAG-Sun1/sfGFP)Nat/J
003255	B6;129-Plp1^tm1Kan/J
034559	B6;129-Prdm2^tm1Shg/J
036525	B6;129-Scgb1a1^tm1(icre)Fjd/J
039020	B6;129-Vgf^tm5Srjs/AbarJ
006374	B6;129P2-Rph3a^tm1Sud/J
021968	B6;129S-Vcp^tm1Itl/J
035714	B6;129S1-Gt(ROSA)26Sor^tm1(Bcor*A)Vjba/J
003284	B6;129S1-Il1rap^tm1Roml/J
036300	B6;129S4-Cux1^tm1(cre/ERT2)Zjh/J
039106	B6;129S6-Ces1d^tm1Reli/J
023399	B6;129S6-Ctbp2^tm1Sud/J
034237	B6;129X-Stx2^tm1Dcru/J
039470	B6;129X1-Nfe2l1^tm1Jefc/J
012705	B6;CBA-Tg(ATXN3*)84.2Cce/IbezJ
006680	B6;CBA-Tg(Olfr16*,taulacZ)19Mom/MomJ
003627	B6C3-Tg(HD82Gln)81Gschi/J
002810	B6CBA-Tg(HDexon1)62Gpb/1J
006494	B6CBA-Tg(HDexon1)62Gpb/3J
037014	B6J.129P2-Pofut2^Gt(RST434)Byg/BchMmjax
027409	B6J.129S1-Htt^tm1Mfc/140ChdiJ
027410	B6J.129S1-Htt^tm1Mfc/190ChdiJ
370476	B6J.129S1-Htt^tm1Mfc/190JChdi
027419	B6J.Cg-Tg(HDexon1)62Gpb/110ChdiJ
027421	B6J.Cg-Tg(HDexon1)62Gpb/160ChdiJ
034731	B6N(129S4)-Srd5a3^tm1c(EUCOMM)Wtsi/J
027971	B6N.129(Cg)-Pax6^tm1Pgr/J
032924	B6N.129X1(B6J)-Msra^tm1Jmo/RlevMmjax
038997	B6N.Cg-Hprt1^tm1(Gdf9-cas9)Jahe/J
027413	B6N.Cg-Htt^tm1Xen/45ChdiJ
027414	B6N.Cg-Htt^tm2Xen/45ChdiJ
027415	B6N.Cg-Htt^tm3Xen/80ChdiJ
027416	B6N.Cg-Htt^tm4Xen/80ChdiJ
027417	B6N.Cg-Htt^tm5Xen/105ChdiJ
027418	B6N.Cg-Htt^tm6Xen/105ChdiJ
017605	C.129S4-Icos^tm1Shr/J
031371	C.129S6-Pglyrp3^tm1Rdz/J
005417	C;129P2-Npy2r^tm1Pern/J
003532	C3HeB.129S7-Kcna1^tm1Tem/J
038023	C57BL/6-Cxcr3^tm2.1Arsa/J
035489	C57BL/6-Elmo1^tm1Mki/J
039508	C57BL/6-Gt(ROSA)26Sor^tm1(CAG-CD63/copGFP)Yoy/J
038452	C57BL/6-Gt(ROSA)26Sor^tm1(CAG-Egfr*)Rnem/J
038249	C57BL/6-Gt(ROSA)26Sor^tm1(CAG-Gria2*)Bli/J
038454	C57BL/6-Gt(ROSA)26Sor^tm2(CAG-Egfr*L860R)Rnem/J
035698	C57BL/6-Il17a^tm1Bcgen Il17f^em1Litt/J
026685	C57BL/6-Nrxn1^tm5Sud/J
017959	C57BL/6-Pml^tm1(PML/RARA)Ley/J
038172	C57BL/6-Rgr^tm1.1Kpal/J
035693	C57BL/6-Tlr9^tm2Gbrt/J
032131	C57BL/6-Tg(Scx-GFP/cre)1Stzr/J
003651	C57BL/6-Tg(Zp3-cre)93Knw/J
038551	C57BL/6J-Gt(ROSA)26Sor^em1(CAG-PACS1*R203W)Gath/J
038552	C57BL/6J-Gt(ROSA)26Sor^em2(CAG-PACS1)Gath/J
035696	C57BL/6J-Saa3^em1Litt/J
023088	C57BL/6J-Tg(C9orf72_i2)8Lutzy/J
023099	C57BL/6J-Tg(C9orf72_i3)112Lutzy/J
027422	D2.Cg-Tg(HDexon1)62Gpb/160ChdiJ
027424	D2.Cg-Tg(HDexon1)62Gpb/240ChdiJ
027425	D2.Cg-Tg(HDexon1)62Gpb/280ChdiJ
002787	DBA/1LacJ-Alox5ap^tm1Jdm/J
004938	FVB-Tg(YAC128)53Hay/J
038293	FVB.Cg-Pde6b^+ Tyr^c-ch Ccdc198^em1Iad/J
013742	FVB.Cg-Slc9a1^tm1Smb/Mmjax
032031	FVB/N-Tg(HSA*LR)20bCath/J
027379	FVB/N-Tg(HTT*)NXwy/J
008197	FVB/N-Tg(HTT*97Q)IXwy/J
027433	FVB/N-Tg(HTT*97Q)LXwy/ChdiJ
036633	FVB/NJ-Tg(C9orf72_i3)101Lutzy/J
037050	FVB/NJ-Tg(HTT*131Q)BACXwy/120J
036631	FVB/NJ-Tg(Ube3a)5Mpan/J
007850	J:NU
039055	NOD.Cg-Prkdc^scid H2-K1^d-em1Dvs H2-Ab1^g7-em1Dvs H2-D1^b-em5Dvs Il2rg^tm1Wjl Tg(HLA-DQA1,HLA-DQB1)1Dv/DvsJ
035970	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(YAC128)53Hay/JnolJ
026014	NOD.Cg-Rag1^tm1Mom Kit^W-41J Il2rg^tm1Wjl/EavJ
017914	NOD.Cg-Rag1^tm1Mom Il2rg^tm1Wjl Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito/ScasJ
007799	NOD.Cg-Rag1^tm1Mom Il2rg^tm1Wjl/SzJ
014568	NOD.Cg-Rag1^tm1Mom Ins2^Akita Il2rg^tm1Wjl/SzJ
035185	STOCK Adgrl1^tm2c(EUCOMM)Hmgu/SudJ
006873	STOCK Cebpb^tm1Vpo/J
038587	STOCK Foxi3^em2(cre/ERT2)Akg/J
031007	STOCK Fxn^em2.1Lutzy Tg(FXN)Y47Pook/J
030395	STOCK Fxn^em2.1Lutzy Tg(FXN)YG8Pook/800J
030930	STOCK Fxn^em2.1Lutzy Tg(FXN)YG8Pook/J
024097	STOCK Fxn^tm1Mkn Tg(FXN)Y47Pook/J
024113	STOCK Fxn^tm1Mkn Tg(FXN)YG8Pook/2J
010963	STOCK Fxn^tm1Mkn Tg(FXN)YG22Pook/J
008398	STOCK Fxn^tm1Mkn Tg(FXN)YG8Pook/J
038382	STOCK Ggta1^tm1Jbl/NbndrJ
038583	STOCK Gt(ROSA)26Sor^em1(CAG-Atoh1/EGFP)Akg/J
038584	STOCK Gt(ROSA)26Sor^em2(CAG-Gf1i,-Atoh1/EGFP)Akg/J
038585	STOCK Gt(ROSA)26Sor^em3(CAG-Gf1i,-Atoh1/EGFP,-Pou4f3)Akg/J
038176	STOCK Gt(ROSA)26Sor^tm1(CAG-APOBEC3B)Rshar/J
037697	STOCK Gt(ROSA)26Sor^tm13(CAG-HRP)Luo/J
038177	STOCK Gt(ROSA)26Sor^tm2(CAG-APOBEC3B*E255A)Rshar/J
012266	STOCK Gt(ROSA)26Sor^tm5(ACTB-tTA)Luo/J
038629	STOCK Gt(ROSA)26Sor^tm65.2(CAG-tdTomato)Hze Pdgfrb^tm1.1(flpo)Leol/LeolJ
003456	STOCK Htt^tm5Mem/J
039204	STOCK Neurog2^tm1Cschu/J
013169	STOCK Nphp1^tm1Jgg/J
035925	STOCK Opn4^tm1(cre)Saha/J
038627	STOCK Pdgfra^tm1(flpo)Leol Gt(ROSA)26Sor^tm65.2(CAG-tdTomato)Hze/LeolJ
037681	STOCK Plk4^em4Ahol/J
006770	STOCK Rag1^tm1Mom Tg(TIE2GFP)287Sato/J
037469	STOCK Sftpa1^tm1Haw Tg(SFTPC-SFTPA1*)T1Jflo/Mmjax
037902	STOCK Sftpc^tm1(dre/ERT2)Bzsh/J
036439	STOCK Slc2a2^tm2.1Thor/J
012940	STOCK Snta1^tm1Scf/J
039126	STOCK Spata7^tm1Mrd/RuchJ
035561	STOCK Tc(HSA21,CAG-EGFP)1Yakaz/J
030324	STOCK Tg(FXN)YG8Pook/J
018299	STOCK Tg(FXN*)1Sars Fxn^tm1Mkn/J
197 strains use this protocol