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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR1770 | TGA CGG GTG TGA CAG CAG TAG | Transgene | A | |||
oIMR1771 | CAG TGG CTG CTG CAA TG | Transgene | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0025 | Fluorophore-1 | CCC TGC TCC CTC CAC TGT CTT CTG G | Quencher-1 | MUT Probe | ||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa Probe Fast QPCR | 1.00 X |
oIMR1544 | 0.40 uM |
oIMR1770 | 0.40 uM |
oIMR1771 | 0.40 uM |
oIMR3580 | 0.40 uM |
Tg Probe | 0.15 uM |
IC Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |
Stock Number | Strain Name |
---|---|
023837 | B6.Cg-Tg(SNCA)OVX37Rwm Sncatm1Rosl/J |
004479 | B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J |
032799 | C57BL/6-Tg(Thy1-SNCA*E35K*E46K*E61K)3798Nuber/J |
018442 | C57BL/6N-Tg(SNCA)129Mjff/J |
017678 | FVB;129-Pink1tm1Aub Tg(Prnp-SNCA*A53T)AAub/J |
010710 | FVB;129S6-Sncatm1Nbm Tg(SNCA)1Nbm/J |
010788 | FVB;129S6-Sncatm1Nbm Tg(SNCA*A30P)1Nbm Tg(SNCA*A30P)2Nbm/J |
010799 | FVB;129S6-Tg(SNCA*A53T)1Nbm Sncatm1Nbm Tg(SNCA*A53T)2Nbm/J |
027227 | STOCK Gba1tm1Rlp Tg(SNCA*A53T)1Nbm Sncatm1Nbm Tg(SNCA*A53T)2Nbm/Mmjax |
037870 | STOCK Sncatm1Rosl Tg(Cnp-SNCA)M2Vle/KlukJ |
10 strains use this protocol |