Stock No: 008355
Protocol 20644: Standard PCR Assay - Slc6a4<tm1Kpl> Alternate1
Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Mutant = ~190 bp
Heterozygote = ~190 bp and 237 bp
Wild type = 237 bp

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note
30801 CCT GTC TGT CAT TGG CTA TGC Wild type Forward
30802 GCT GAC TGG AGA TCA GGC TA Common
oIMR6773 TGG CTA CCC GTG ATA TTG CT Mutant Forward

Strains Using This Protocol

This is the only strain that uses this protocol.