Protocol 21006: Separated PCR Assay - GAL Panel-B6J vs. 129S

B6.129X1-Nfe2l2^tm1Ywk/J

Stock No: 017009

Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note

Strains Using This Protocol

Stock Number	Strain Name
035435	B6(129S4)-Xcr1^tm1.1(cre)Kmm/J
017009	B6.129X1-Nfe2l2^tm1Ywk/J
008590	B6;129P2-Cxcl14^tm1Litt/J
006381	B6;129S-Stxbp1^tm1Sud/J
027992	B6J.129S7(Cg)-Mir22^tm1.1Arod/J
028867	B6J.Cg-Gad2^tm2(cre)Zjh/MwarJ
010802	STOCK Gad2^tm2(cre)Zjh/J
7 strains use this protocol