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TGS Design, KC 12/20/11.
MUT = GG
WT = GGG
amplicon = 128 bp
Foxn1 genomic
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
13775 | CTT CCG CCT TTC TCC TTC AG | Forward | A | |||
13776 | CCT CAT GGA AGT GCC TCT TG | Reverse | A | |||
13777 | Fluorophore-1 | CAG CCC AGG CGC TTC | Quencher-1 | MUT Probe | ||
13778 | Fluorophore-2 | CAG CCC AGG GCG CTT C | Quencher-2 | WT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
13775 | 0.40 uM |
13776 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |