For in-depth product & services help, ask our
Technical Information Scientists
Mut=T/T (H2<d>)
WT= A/A (other haplotypes)
Aaatactgatcatgcaatggctgggc(A/T)gaagagagaata
gggtgGGACATCTGCCAATCA
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
21926 | Fluorophore-1 | ATG GCT GGG CAG AAG AGA G | Quencher-1 | WT Probe | ||
21928 | Fluorophore-2 | ATG GCT GGG CTG AAG AGA G | Quencher-2 | MUT Probe | ||
22202 | CCA AGA ACC TCC TCT CTC ATG T | Forward | A | |||
22203 | GCA GAT GTC CCA CCC TAT TC | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
22202 | 0.40 uM |
22203 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
027528 | B10.Cg-H2d Tg(Alb1-HBV)66Bri/ChiJ |
000462 | B10.D2-H2d/n2SnJ |
000461 | B10.D2-Hc0 H2d H2-T18c/oSnJ |
000463 | B10.D2-Hc1 H2d H2-T18c/nSnJ |
000651 | BALB/cJ |
002845 | C.B-H2b Tg(H2-Dd)D8Gja/LilJ |
000666 | C57BL/10SnJ |
004353 | C57BL/6-Tg(UBC-GFP)30Scha/J |
007076 | CByJ.B6-Tg(UBC-GFP)30Scha H2b/J |
034677 | CByJ.B6-Tg(UBC-GFP)30Scha/J |
028062 | STOCK Ptprca TcrbLn1Bdb TcraLn1Bdb H2d/J |
11 strains use this protocol |