For in-depth product & services help, ask our
Technical Information Scientists
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR0461 | CTG TGT CTG TAT GAC CCA CC | Wild type Forward | A | |||
oIMR0462 | CAG GAG TTT CCT AAG CAA CG | Wild type Reverse | A | |||
oIMR6916 | CTT GGG TGG AGA GGC TAT TC | Mutant Forward | A | Neo F | ||
oIMR6917 | AGG TGA GAT GAC AGG AGA TC | Mutant Reverse | A | Neo R |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTP KAPA | 0.26 mM |
oIMR0461 | 0.50 uM |
oIMR0462 | 0.50 uM |
oIMR6916 | 0.50 uM |
oIMR6917 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
We use cookies to personalize our website and to analyze web traffic to improve the user experience. You may decline these cookies although certain areas of the site may not function without them. Please refer to our privacy policy for more information.