For in-depth product & services help, ask our
Technical Information Scientists
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1678 | GTA AAT AAT TGT GCT TCG TCA G | Common | A | |||
oIMR1679 | TAG AAA GGT GCA CGG GTG TG | Mutant | A | |||
oIMR1680 | CAA ATC TAG GCA TTA ACA GTG | Wild type | A |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTP KAPA | 0.26 mM |
oIMR1678 | 0.50 uM |
oIMR1679 | 0.50 uM |
oIMR1680 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
000482 | B6.MRL-Faslpr/J |
000480 | C3.MRL-Faslpr/J |
002455 | MRL.Cg-B2mtm1Unc Faslpr |
000486 | MRL/MpJ |
003896 | MRL/MpJ Faslpr-Foxq1sa-J/J |
006825 | MRL/MpJ-Faslpr/2J |
000485 | MRL/MpJ-Faslpr/J |
004519 | NOD.MRL(C3)-Faslpr/DoiJ |
004922 | NOD.MRL-Faslpr/Dvs |
9 strains use this protocol |