For in-depth product & services help, ask our
Technical Information Scientists
Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate instrument specific Fluorophore/Quencher combination.
Mutant= 110 bp
Wild Type = 77 bp
X-linked
>chrX:101267366-101267442 77bp CCAAAGAGATTACTTCTGGCTGT CTGGAGCTGGACAACAAATG
328 bp deletion
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
48598 | CCA AAG AGA TTA CTT CTG GCT GT | Wild type Forward | A | |||
48599 | CTG GAG CTG GAC AAC AAA TG | Wild type Reverse | A | |||
48600 | Fluorophore-1 | AGA TAC AAA AAG AAG ATA TCC AGC TCT AC | Quencher-1 | WT Probe | ||
48601 | AGC TCT TCA GGA ACC CTA CCA | Mutant Forward | A | |||
48602 | CCC TTA TCC CTC CTC CCT TC | Mutant Reverse | A | |||
48603 | Fluorophore-2 | CAG ACC AGA TGC AGA AAA GAG AAG | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
48598 | 0.40 uM |
48599 | 0.40 uM |
48601 | 0.40 uM |
48602 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |