APOE R158C REV/REV "ɛ2" (B)

Product Code: JIPSC1166

Background

Genetics

Gene

APOE

Condition

Alzheimer disease

Allele Charactertistics

Recessive

Other names in ClinVar

R148C, R176C, R202C, APOE2-determining SNP

View Information in ClinVar

Derived from Cell Line

JIPSC1146_APOE_R158C_SNV/SNV (B)

Genomic Features

Genomic Coordinate (GRCh38)

chr19:44908822 (+)

dbSNP

rs7412

Genomic Sequence

CTCCTCCGCGATGCCGATGACCTGCAGAAG[C/T]GCCTGGCAGTGTACCAGGCCGGGGCCCGCG
View With Genome Browser

Gene Editing Reagents

Nuclease

SpCas9

Protospacer Sequence

CGATGACCTGCAGAAGTGCC

Donor Template

CTCGCGGATGGCGCTGAGGCCGCGCTCGGCGCCCTCGCGGGCCCCGGCCTGGTACACTGCCAGGCGCTTCTGCAGGTCATCGGCATCGCGGAGGAGCCGC

DNA Primers for Sequence Validation

PCR Forward

CTGGAGGAACAACTGACCCC

PCR Reverse

GCTGCCCATCTCCTCCATC

Sanger Sequencing Primer