GRN Q125X REV/REV

Product Code: JIPSC1170

Background

Genetics

Gene

GRN

Condition

Frontotemporal dementia

Allele Charactertistics

Autosomal Dominant

View Information in ClinVar

Derived from Cell Line

JIPSC1060_GRN_Q125X_SNV/SNV

Genomic Features

Genomic Coordinate (GRCh38)

chr13:76992176 (+)

dbSNP

rs63750077

Genomic Sequence

TCCACAGGTAACAACTCCGTGGGTGCCATC[C/T]AGTGCCCTGATAGTCAGTTCGAATGCCCGG
View With Genome Browser

Gene Editing Reagents

Nuclease

SpCas9

Protospacer Sequence

CTGACTATCAGGGCACTAGA

Donor Template

CCTGAGTGGGCTGGTAGTATCCTGGGTCATCTTGTCCACAGGTAACAACTCCGTGGGTGCCATCCAGTGCCCTGATAGTCAGTTCGAATGCCCGGACTTC

DNA Primers for Sequence Validation

PCR Forward

GAGCTGGGCCGGTCTAATAC

PCR Reverse

TGGTCCTGACTCCGTCTTCA

Sanger Sequencing Primer