For in-depth product & services help, ask our
Technical Information Scientists
Mutant = ~550 bp
Heterozygote = 318 bp and ~550 bp
Wild type = 318 bp
>chr5:108819374+108819691 318bp CATGTCCTACAGCCCCTCTC ACCATTTGCAAGGAAAGCAC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
36758 | CAT GTC CTA CAG CCC CTC TC | Common | A | |||
36759 | ACC ATT TGC AAG GAA AGC AC | Wild type Reverse | A | |||
oIMR2093 | AAG CTA GCT GCA GTA ACG CCA TTT | Mutant Reverse | A |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTP KAPA | 0.26 mM |
36758 | 0.50 uM |
36759 | 0.50 uM |
oIMR2093 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
037206 | B6.129-Optntm1.1Htse/J |
004202 | B6.C3 Pde6brd1 Hps4le/+ +-Lmx1adr-8J/J |
000002 | B6.C3-Pde6brd1 Hps4le/J |
015850 | B6.Cg-Pde6b+ Tg(Rho-icre)1Ck/Boc |
006576 | B6.FVB-Tg(GNAT2-Dta)98Wwk/J |
004462 | B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax |
028590 | B6C3-Tg(tetO/Prnp-TARDBP*)2Vle/J |
000235 | B6C3Fe a/a-Relnrl/J |
002810 | B6CBA-Tg(HDexon1)62Gpb/1J |
001924 | B6EiC3Sn a/A-Ts(1716)65Dn/J |
004850 | B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDnJ |
006554 | B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax |
001957 | C3A Pde6brd1.O20/A-Prph2Rd2/J |
001912 | C3A.BLiA-Pde6b+/J |
001979 | C3A.Cg-Pde6b+ Prph2Rd2/J |
024703 | C3A.Cg-Pde6b+Tg(Thy1-CFP)23Jrs/SjJ |
003648 | C3Sn.BLiA-Pde6b+/DnJ |
000654 | CBA/CaJ |
000656 | CBA/J |
034245 | FVB(129P2)-Pde6b+ Gtf2iem2Jdd Gtf2ird1em2Jdd Tyrc-ch/Mmjax |
034672 | FVB(129P2)-Pde6b+ Gtf2ird1em3Jdd Tyrc-ch/Mmjax |
004624 | FVB.129P2-Pde6b+ Tyrc-ch Fmr1tm1Cgr/J |
004828 | FVB.129P2-Pde6b+ Tyrc-ch/AntJ |
022880 | FVB.Cg-Pde6b+ Lrrk1tm1.1Mjff Tyrc-ch/J |
038293 | FVB.Cg-Pde6b+ Tyrc-ch Ccdc198em1Iad/J |
003342 | STOCK Hbatm1Paz Hbbtm1Tow Tg(HBA-HBBs)41Paz/J |
013169 | STOCK Nphp1tm1Jgg/J |
005441 | STOCK Tg(CAG-DsRed*MST)1Nagy/J |
004808 | STOCK Tg(MAPT)8cPdav Mapttm1(EGFP)Klt/J |
010815 | STOCK Tg(mI56i-flpe)39Fsh/J |
30 strains use this protocol |