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Transgene Tm = 83°C +/- 1.0°C
Internal positive control Tm = 86.5°C+/- 1.0°C
Transgene = ~100 bp
Internal positive control = 324 bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1084 | GCG GTC TGG CAG TAA AAA CTA TC | Transgene Forward | A | Cre F | ||
oIMR1085 | GTG AAA CAG CAT TGC TGT CAC TT | Transgene Reverse | A | Cre R | ||
oIMR7338 | CTA GGC CAC AGA ATT GAA AGA TCT | Internal Positive Control Forward | A | |||
oIMR7339 | GTA GGT GGA AAT TCT AGC ATC ATC C | Internal Positive Control Reverse | A |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTP KAPA | 0.26 mM |
oIMR1084 | 0.50 uM |
oIMR1085 | 0.50 uM |
oIMR7338 | 0.50 uM |
oIMR7339 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
005989 | 129;FVB-Tg(PTH-cre)4167Slib/J |
017789 | 129S-Slc11a2tm2Nca/J |
007179 | 129S.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/J |
007915 | 129S.FVB-Tg(Amh-cre)8815Reb/J |
004302 | 129S1/Sv-Hprt1tm1(CAG-cre)Mnn/J |
008523 | 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ |
032051 | B6(129S4)-Kittm1.1(cre)Jmol/J |
037511 | B6(129S4)-Pou2f3tm1.1(cre/ERT2)Imt/J |
024976 | B6(129S4)-Tspotm1.1Maf/J |
009575 | B6(129S4)-Et(cre/ERT2)119Rdav/J |
009580 | B6(129S4)-Et(cre/ERT2)1382Rdav/J |
009581 | B6(129S4)-Et(cre/ERT2)1642Rdav/J |
009582 | B6(129S4)-Et(cre/ERT2)1645Rdav/J |
009583 | B6(129S4)-Et(cre/ERT2)1957Rdav/J |
009584 | B6(129S4)-Et(cre/ERT2)2007Rdav/J |
009585 | B6(129S4)-Et(cre/ERT2)2047Rdav/J |
009574 | B6(129S4)-Et(cre/ERT2)21Rdav/J |
009577 | B6(129S4)-Et(cre/ERT2)296Rdav/J |
009578 | B6(129S4)-Et(cre/ERT2)398Rdav/J |
009573 | B6(129S4)-Et(cre/ERT2)4Rdav/J |
009579 | B6(129S4)-Et(cre/ERT2)837Rdav/J |
038514 | B6(129S6)-Tg(Slc6a5-cre/ERT2)#Uze/J |
039195 | B6(Cg)-Il17atm1.1(EYFP/cre)Ehs/J |
026512 | B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J |
021012 | B6(D2)-Tg(CAG-Brainbow1.0)3Eggn/J |
037052 | B6(FVB)-Tg(Sprr2f-cre)2Dcas/J |
018057 | B6.129(Cg)-Cul7tm2.1Jdec/J |
008320 | B6.129(Cg)-Leprtm2(cre)Rck/J |
033964 | B6.129(Cg)-Ptf1atm2(cre/ESR1)Cvw Krastm4Tyj Ptentm1Hwu/J |
036564 | B6.129(Cg)-Scn10atm2(cre)Jwo/TjpJ |
008463 | B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J |
018445 | B6.129-Mre11atm1.1Rchd/J |
025567 | B6.129-Olig2tm1.1(cre)Wdr/J |
005697 | B6.129-Otx1tm4(cre)Asim/J |
004146 | B6.129-Tg(Pcp2-cre)2Mpin/J |
021160 | B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2)Litt/WganJ |
018054 | B6.129P2(Cg)-Pitx2tm1.1Dmm/J |
023726 | B6.129P2-C1qatm1.1Tenn/J |
016934 | B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J |
018997 | B6.129S(FVB)-Lhx3tm1.1Sjr/J |
016132 | B6.129S4-Perptm2Att/J |
006447 | B6.129S6(CBA)-Cebpatm1Dgt/J |
012900 | B6.129S6-Stat5btm1Mam Stat5atm2Mam/Mmjax |
008601 | B6.Cg-7630403G23RikTg(Th-cre)1Tmd/J |
006230 | B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J |
036450 | B6.Cg-Foxl1tm2(cre/ERT2)Khk/Mmjax |
009107 | B6.Cg-H2az2Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J |
032923 | B6.Cg-Kittm1(cre)Htng/J |
028536 | B6.Cg-Ndnftm1.1(folA/cre)Hze/J |
030544 | B6.Cg-Npy1rtm1.1(cre/GFP)Rpa/J |
031485 | B6.Cg-Pou4f2tm1(cre)Bnt/SjJ |
038835 | B6.Cg-Sdk1em1(cre/GFP)Jrs/KrishJ |
017889 | B6.Cg-Shank3tm1.1Bux/J |
022865 | B6.Cg-Trib2tm1.1(cre/ERT2)Hze/J |
017346 | B6.Cg-Tg(A930038C07Rik-cre)1Aibs/J |
006149 | B6.Cg-Tg(ACTA1-cre)79Jme/J |
030215 | B6.Cg-Tg(Amigo2-cre)1Sieg/J |
009352 | B6.Cg-Tg(CDX2-cre*)189Erf/J |
006368 | B6.Cg-Tg(Cr2-cre)3Cgn/J |
029594 | B6.Cg-Tg(Dmp1-cre/ERT2)D77Pdp/J |
006663 | B6.Cg-Tg(Eno2-cre)39Jme/J |
035668 | B6.Cg-Tg(Gata1-cre)1Sho/MdfJ |
009642 | B6.Cg-Tg(GH1-cre)1Sac/J |
003573 | B6.Cg-Tg(Ins2-cre)25Mgn/J |
005584 | B6.Cg-Tg(Prrx1-cre)1Cjt/J |
003967 | B6.Cg-Tg(Rbp3-cre)528Jxm/J |
021614 | B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J |
004128 | B6.Cg-Tg(Tek-cre)12Flv/J |
008863 | B6.Cg-Tg(Tek-cre)1Ywa/J |
006234 | B6.Cg-Tg(tetO-cre)1Jaw/J |
012328 | B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J |
035670 | B6.Cg-Tg(VAV1-cre)1Graf/MdfJ |
020282 | B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J |
006451 | B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J |
006137 | B6.FVB-Tg(Cdh5-cre)7Mlia/J |
018980 | B6.FVB-Tg(Ddx4-cre)1Dcas/KnwJ |
003724 | B6.FVB-Tg(EIIa-cre)C5379Lmgd/J |
011069 | B6.FVB-Tg(Gh1-cre)bKnmn/J |
011038 | B6.FVB-Tg(Myh6-cre)2182Mds/J |
010714 | B6.FVB-Tg(Pomc-cre)1Lowl/J |
003394 | B6.FVB-Tg(Zp3-cre)3Mrt/J |
017601 | B6;129-Eif2s1tm1Rjk/J |
016210 | B6;129-Lrrk2tm3.1Shn/J |
018319 | B6;129-Plxnd1tm1.1Tmj/J |
009600 | B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J |
018773 | B6;129-Sult2b1tm1Rus/J |
022864 | B6;129S-Rasgrf2tm1(cre/folA)Hze/J |
017593 | B6;129S-Sox2tm1(cre/ERT2)Hoch/J |
009388 | B6;129S1-Osr2tm2(cre)Jian/J |
036300 | B6;129S4-Cux1tm1(cre/ERT2)Zjh/J |
018019 | B6;129S4-Srsf2tm1Xdfu/J |
009576 | B6;129S4-Et(cre/ERT2)278Rdav/J |
017495 | B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J |
018937 | B6;C-Tg(Erv4-cre/ERT2*)657Slp/J |
038989 | B6;C3-Oxtrem4(icre/ERT2)Yinn/2J |
009616 | B6;C3-Tg(A930038C07Rik-cre)4Aibs/J |
008844 | B6;C3-Tg(Ctgf-cre)2Aibs/J |
008839 | B6;C3-Tg(Cyp39a1-cre)1Aibs/J |
009117 | B6;C3-Tg(Cyp39a1-cre)7Aibs/J |
009111 | B6;C3-Tg(Scnn1a-cre)1Aibs/J |
009112 | B6;C3-Tg(Scnn1a-cre)2Aibs/J |
009613 | B6;C3-Tg(Scnn1a-cre)3Aibs/J |
009103 | B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J |
025809 | B6;CBA-Tg(Fgfr3-icre/ERT2)4-2Wdr/J |
024926 | B6;D2-Tg(Fshr-cre)1Ldu/J |
003466 | B6;D2-Tg(Sycp1-cre)4Min/J |
015855 | B6;DBA-Tg(Upk3a-GFP/cre/ERT2)26Amc/J |
018402 | B6;FVB-Nrn1tm1.2Ndiv/J |
023748 | B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J |
003734 | B6;FVB-Tg(GZMB-cre)1Jcb/J |
004426 | B6;SJL-Tg(Cga-cre)3Sac/J |
003554 | B6;SJL-Tg(Col2a1-cre)1Bhr/J |
017738 | B6;SJL-Tg(Foxl1-cre)1Khk/J |
005249 | B6;SJL-Tg(Krt1-15-cre/PGR*)22Cot/J |
003552 | B6129-Tg(Wap-cre)11738Mam/J |
007252 | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ |
017741 | B6N.129(Cg)-Mecp2tm1.1Joez/J |
017704 | B6N.129-Pik3catm1Jjz/J |
016881 | B6N.Cg-Adgra2tm1.1Bstc/J |
019102 | B6N.Cg-Tg(CAG-cre/Esr1*)5Amc/CjDswJ |
018966 | B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J |
018965 | B6N.Cg-Tg(Fabp4-cre)1Rev/J |
018960 | B6N.Cg-Tg(Ins2-cre)25Mgn/J |
018964 | B6N.Cg-Tg(KRT14-cre)1Amc/J |
019103 | B6N.Cg-Tg(Nes-cre)1Kln/CjDswJ |
003465 | BALB/c-Tg(CMV-cre)1Cgn/J |
013756 | C.129S-Batf3tm1Kmm/J |
004126 | C.Cg-Cd19tm1(cre)Cgn Ighb/J |
005673 | C.Cg-Tg(Mx1-cre)1Cgn/J |
006244 | C.Cg-Tg(tetO-cre)1Jaw/J |
009155 | C57BL/6-Cldn6tm1(cre)Dkwu/J |
035489 | C57BL/6-Elmo1tm1Mki/J |
038053 | C57BL/6-Oprm1tm2.1(EGFP/cre)Ics/KffJ |
016097 | C57BL/6-Tg(Car1-cre)5Flt/J |
008870 | C57BL/6-Tg(Hspa2-cre)1Eddy/J |
024034 | C57BL/6-Tg(Pmch-cre)1Rck/J |
032131 | C57BL/6-Tg(Scx-GFP/cre)1Stzr/J |
036429 | C57BL/6J-Gnetm1Dar/J |
032080 | C57BL/6J-Tg(Cd8a*-cre)B8Asin/J |
018899 | C57BL/6J-Tg(Krt6,-cre,-Cerulean)5Grsr/Grsr |
021582 | C57BL/6J-Tg(Mchr1-cre)1Emf/J |
022883 | C57BL/6J-Tg(Six6-cre)3Grsr/GrsrJ |
027789 | C57BL/6N-Fgd5tm3(cre/ERT2)Djr/J |
023453 | C57BL/6N-Lhx8tm1(cre)Grsr/2GrsrJ |
012686 | C57BL/6N-Tg(Ppp1r2-cre)4127Nkza/J |
012929 | FVB(Cg)-Tg(Dhh-cre)1Mejr/J |
004600 | FVB-Tg(GFAP-cre)25Mes/J |
005025 | FVB.Cg-Grm7Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb/J |
006139 | FVB.Cg-Tg(ACTA1-cre)79Jme/J |
006297 | FVB.Cg-Tg(Eno2-cre)39Jme/J |
018394 | FVB.Cg-Tg(KRT5-cre/ERT2)2Ipc/JeldJ |
008244 | FVB.Cg-Tg(tetO-cre)1Jaw/J |
003314 | FVB/N-Tg(EIIa-cre)C5379Lmgd/J |
003377 | FVB/N-Tg(Zp3-cre)3Mrt/J |
017678 | FVB;129-Pink1tm1Aub Tg(Prnp-SNCA*A53T)AAub/J |
023325 | FVB;B6-Tg(Pbsn-cre)20Fwan/J |
034859 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CAG-cre/Esr1*)5Amc/J |
034795 | NOD.Cg-Tg(CAG-cre/Esr1*)5Amc/J |
013251 | NOD.FVB-Tg(EIIa-cre)C5379Lmgd/J |
004986 | NOD/ShiLt-Tg(Ins2-cre)3Lt/LtJ |
003855 | NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ |
035185 | STOCK Adgrl1tm2c(EUCOMM)Hmgu/SudJ |
036942 | STOCK Agertm2.1(cre/ERT2)Blh/2J |
039535 | STOCK Aldh1a3tm1(cre/ERT2)Dac/J |
037028 | STOCK Calcrtm1.1(cre)Mgmj/J |
007916 | STOCK En1tm2(cre)Wrst/J |
007913 | STOCK Gli1tm3(cre/ERT2)Alj/J |
008783 | STOCK Grm7Tg(SMN2)89Ahmb Smn1tm3(SMN2/Smn1)Mrph Tg(CAG-cre/Esr1*)5Amc Tg(SMN2*delta7)4299Ahmb/J |
022793 | STOCK Gt(ROSA)26Sortm1(LRRK2*R1441C)Djmo/J |
003829 | STOCK H2az2Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J |
017298 | STOCK Hmgn5tm1.1Mbus/J |
035978 | STOCK Hoxb8tm2.1(cre)Mrc/J |
032429 | STOCK Krastm4Tyj Trp53tm1Brn Tg(Pdx1-cre/Esr1*)#Dam/J |
018418 | STOCK Lrig1tm1.1(cre/ERT2)Rjc/J |
016945 | STOCK Mir138-1tm1Mtm/Mmjax |
004192 | STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J |
037139 | STOCK Myl1tm1(cre)Sjb Atg7tm1Tchi Gaatm1Rabn/PuroMmjax |
035303 | STOCK Obscntm1Chen/J |
035925 | STOCK Opn4tm1(cre)Saha/J |
035045 | STOCK Oprk1tm1.1(cre)Sros/J |
030543 | STOCK Oxtrtm1(cre/GFP)Rpa/J |
022972 | STOCK Pros1tm1Grl/J |
019378 | STOCK Ptf1atm2(cre/ESR1)Cvw/J |
013086 | STOCK Rxratm1Krc/J |
020080 | STOCK Slc6a3tm1(cre)Xz/J |
023405 | STOCK Tfap2atm1Hsv/J |
036906 | STOCK Tmem178btm1c(KOMP)Wtsi Tmem178tm1.1Sud/J |
010911 | STOCK Wt1tm1(EGFP/cre)Wtp/J |
007684 | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J |
004453 | STOCK Tg(CAG-cre/Esr1*)5Amc/J |
035566 | STOCK Tg(CAG-mRuby2*,-mClover*)1Srgt/J |
009615 | STOCK Tg(Cartpt-cre)1Aibs/J |
017336 | STOCK Tg(Cd4-cre)1Cwi/BfluJ |
005105 | STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J |
008852 | STOCK Tg(En2-cre)22Alj/J |
005938 | STOCK Tg(Eno2-cre)39Jme/J |
022763 | STOCK Tg(Eno2-cre/ERT2)1Pohlk/J |
027681 | STOCK Tg(Hnf1b-cre/ERT2)1Jfer/J |
019380 | STOCK Tg(IVL-cre/ERT2)1Blpn/J |
008582 | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ |
005107 | STOCK Tg(KRT14-cre/ERT)20Efu/J |
017948 | STOCK Tg(KRT18-cre/ERT2)23Blpn/J |
009643 | STOCK Tg(Lhb-cre)1Sac/J |
023724 | STOCK Tg(mI56i-cre,EGFP)1Kc/J |
003553 | STOCK Tg(MMTV-cre)4Mam/J |
008847 | STOCK Tg(Msx2-cre/ERT)27Alj/J |
009074 | STOCK Tg(Myh6-cre)1Jmk/J |
009102 | STOCK Tg(Nefh-cre)12Kul/J |
028364 | STOCK Tg(Neurod1-cre)1Able/J |
005667 | STOCK Tg(Neurog3-cre)C1Able/J |
006207 | STOCK Tg(Pcp2-cre)1Amc/J |
009606 | STOCK Tg(Six2-EGFP/cre)1Amc/J |
032646 | STOCK Tg(Spo11-cre)1Rsw/PecoJ |
006224 | STOCK Tg(tetO-cre)1Jaw/J |
024240 | STOCK Tg(Tnnt2-cre)5Blh/JiaoJ |
008851 | STOCK Tg(Wnt1-cre/ERT)1Alj/J |
018281 | STOCK Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax |
217 strains use this protocol |