Optogenetics Strains

JAX#	COMMON NAME	PROMOTER	EFFECTOR	EXPRESSION	STRAIN NOMENCLATURE (Oct 2019)

Cre-Inducible Lines					STRAIN NOMENCLATURE
012735	Ai35D	R26 :: CAG	Arch / GFP	Cre-dependent ; opsin/xFP	B6;129S-Gt(ROSA)26Sor^{tm35.1(CAG-aop3/GFP)Hz}^e/J
021188	Ai40D	R26 :: CAG	ArchT / EGFP	Cre-dependent ; opsin/xFP	B6.Cg-Gt(ROSA)26Sor^{tm40.1(CAG-aop3/EGFP)Hze}/J
017455	2xChETA-tdT	R26 :: CAG	ChETA/ChR2*H134R and tdTom	Cre-dependent ; opsin/xFP	B6;129-Gt(ROSA)26Sor^{tm1(CAG-COP4E123TH134R,-tdTomato)Gfng}/J
024109	Ai32	R26 :: CAG	ChR2*H134R / EYFP	Cre-dependent ; opsin/xFP	B6.Cg-Gt(ROSA)26Sor^{tm32(CAG-COP4*H134R/EYFP)Hze}/J
012567	Ai27D	R26 :: CAG	hChR2*H134R / tdTomato	Cre-dependent ; opsin/xFP	B6.Cg-Gt(ROSA)26Sor^{tm27.1(CAG-COP4*H134R/tdTomato)Hze}/J
033089	R26-CAG-LNL-GtACR1-ts-FRed-Kv2.1	R26 :: CAG	GtACR1 / FusionRed	Cre-dependent ; opsin/xFP [soma]	B6;129S6-Gt(ROSA)26Sor^tm3Ksvo/J
026294	Rosa26 CAG-LSL-ReaChR-mCit	R26 :: CAG	ReaChR / mCitrine	Cre-dependent ; opsin/xFP	B6.Cg-Gt(ROSA)26Sor^tm2.2Ksvo/J
014539	Ai39	R26 :: CAG	eNpHR3.0 / EYFP	Cre-dependent ; opsin/xFP	B6;129S-Gt(ROSA)26Sor^{tm39(CAG-hop/EYFP)Hze}/J
034240	R26-CAG-lsl-CaMPARI2	R26 :: CAG	CaMPARI2	Cre-dependent ; calcium indicator / photoconvertible xFP	B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-Eos/Calm)Mrc}/J
024477	PC::G5-tdT	RNA pol II :: CAG	GCaMP5G and tdTomato	Cre-dependent ; calcium indicator / xFP	B6;129S6-Polr2a^{Tn(pb-CAG-GCaMP5g,-tdTomato)Tvrd}/J
028865	Ai95D (C57BL/6J)	R26 :: CAG	GCaMP6f	Cre-dependent ; calcium indicator	B6J.Cg-Gt(ROSA)26Sor^{tm95.1(CAG-GCaMP6f)Hze}/MwarJ
029626	R26-Lck-GCaMP6f ^flox	R26 :: CAG	GCaMP6f [membrane]	Cre-dependent ; calcium indicator [membrane]	C57BL/6N-Gt(ROSA)26Sor^{tm1(CAG-GCaMP6f)Khakh}/J
031968	LSL-Salsa6f	R26 :: CAG	GCaMP6f / tdTom (Salsa6f)	Cre-dependent ; ratiometric calcium indicator [green/red]	B6(129S4)-Gt(ROSA)26Sor^{tm1.1(CAG-tdTomato/GCaMP6f)Mdcah}/J
028866	Ai96 (C57BL/6J)	R26 :: CAG	GCaMP6s	Cre-dependent ; calcium indicator	B6J.Cg-Gt(ROSA)26Sor^{tm96(CAG-GCaMP6s)Hze}/MwarJ
031298	iMb2-Control-Mosaic:: iChr2-Control-Mosaic	R26 :: CAG	Mb2-mYFP*A206K ; Mb2-tdTomato ; Mb2-mTFP1 :: H2B-mCherry ; H2B-EGFP ; H2B-mCerulean	Cre-dependent ; mosiac xFP [membrane or chromatin/nuclear]	STOCK Gt(ROSA)26Sor^{tm1(CAG-EYFP,-mEYFP,-tdTomato,-mTFP1)Ben}/Gt(ROSA)26Sor^{tm2(CAG-EYFP,-mCherry,-EGFP,-mCerulean)Ben}/J
031301	iMb2-Control-Mosaic	R26 :: CAG	Mb2-mYFP*A206K ; Mb2-tdTomato ; Mb2-mTFP1	Cre-dependent ; mosiac xFP [membrane]	STOCK Gt(ROSA)26Sor^{tm1(CAG-EYFP,-mEYFP,-tdTomato,-mTFP1)Ben}/J
031302	iChr2-Control-Mosaic	R26 :: CAG	H2B-mCherry ; H2B-EGFP ; H2B-mCerulean	Cre-dependent ; mosiac xFP [chromatin/nuclear]	STOCK Gt(ROSA)26Sor^{tm2(CAG-EYFP,-mCherry,-EGFP,-mCerulean)Ben}/J
032068	Rosa26^LSL-INDIA	R26 :: CAG	INDIA (mNeonGreen-aCasp3-mRuby2)	Cre-dependent ; fluorescent apoptosis reporter	B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz}/J
031776	TB (tTA2-BFP)	H11 :: CAG	H2B-mTagBFP + tTA2	Cre-dependent xFP [nuclear] and tTA	B6;129-Igs2^{tm5(CAG-tTA2,-TagBFP)Luo}/J
029633	Rosa26-CAGs-LSL-RIK	R26 :: CAG	mKate2 + rtTA3	Cre-dependent xFP and rtTA	B6.Cg-Gt(ROSA)26Sor^{tm2(CAG-rtTA3,-mKate2)Slowe}/J
032205	CAMPER	R26 :: CAG	^TEpac^VV (cAMP FRET sensor)	Cre-dependent ; fluorescent cAMP reporter	C57BL/6-Gt(ROSA)26Sor^{tm1(CAG-ECFP/Rapgef3/Venus)Kama}/J
030952	CAG-Sun1/sfGFP	R26 :: CAG	SunTag / superfolder GFP	Cre-dependent ; inner nuclear membrane tag	B6.129-Gt(ROSA)26Sor^{tm5(CAG-Sun1/sfGFP)Nat}/MmbeJ
031925	Rosa26-LSL-dCas9/SunTag	R26 :: CAG	SunTag(BFP) / dCas9	Cre-dependent ; dCas9 and xFP	STOCK Gt(ROSA)26Sor^{tm1(CAG-cas9*,-BFP)Khk}/Mmjax
031645	SPH	CAG	SunTag-p65-HSF1	Cre-dependent ; dCas9 and xFP	B6;D2-Gm33925^{Tn(pb-CAG-cas9*,-EGFP)1Yangh}/J
012570	Ai34D	R26 :: CAG	Synaptophysin / tdTomato	Cre-dependent ; xFP [synapse]	B6;129S-Gt(ROSA)26Sor^{tm34.1(CAG-Syp/tdTomato)Hze}/J
007914	Ai14	R26 :: CAG	tdTomato	Cre-dependent ; xFP	B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J

Cre:Tet-Inducible Lines from Allen Institute					STRAIN NOMENCLATURE
024108	Ai93D;CaMK2a-tTA	TIGRE :: TRE ; CaMK2a	GCaMP6f + tTA	forebrain calcium indicator before dox	STOCK Igs7^{tm93.1(tetO-GCaMP6f)Hze} Tg(Camk2a-tTA)1Mmay/J
031562	Ai162D	TIGRE :: TRE2 + CAG	GCaMP6s + tTA2s	Cre- and Tet-dependent ; calcium indicator	B6.Cg-Igs7^{tm162.1(tetO-GCaMP6s,CAG-tTA2)Hze}/J
030328	Ai148D	TIGRE :: TRE2 + CAG	GCaMP6f + tTA2	Cre- and Tet-dependent ; calcium indicator	B6.Cg-Igs7^{tm148.1(tetO-GCaMP6f,CAG-tTA2)Hze}/J
024115	Ai94D;CaMK2a-tTA	TIGRE :: TRE ; CaMK2a	GCaMP6s + tTA	forebrain calcium indicator before dox	B6.Cg-Igs7^{tm94.1(tetO-GCaMP6s)Hze} Tg(Camk2a-tTA)1Mmay/J
030217	Ai143D	TIGRE :: TRE	RCaMP1.07	Cre- and Tet-dependent ; calcium indicator [red]	B6.Cg-Igs7^{tm143.1(tetO-RCaMP1.07)Hze}/J
030219	Ai139D	TIGRE :: TRE2 + CAG	EGFP + tdT + tTA2	Cre- and Tet-dependent ; differential xFP	B6.Cg-Igs7^{tm139.1(tetO-EGFP,CAG-tdTomato,-tTA2)Hze}/J
030220	Ai140D	TIGRE :: TRE2 + CAG	EGFP + tTA2	Cre- and Tet-dependent ; xFP	B6.Cg-Igs7^{tm140.1(tetO-EGFP,CAG-tTA2)Hze}/J

Cre:FLP Dual Inducible Lines					STRAIN NOMENCLATURE
025109	Ai80D	R26 :: CAG	CatCh (ChR2*L132C) / EYFP	Cre- and FLP-dependent ; opsin/xFP	B6.Cg-Gt(ROSA)26Sor^{tm80.1(CAG-COP4*L132C/EYFP)Hze}/J
024846	Rosa26 CAG-FSF-LSL-ReaChR-mCit	R26 :: CAG	ReaChR / mCitrine	Cre- and FLP-dependent ; opsin/xFP	B6;129S-Gt(ROSA)26Sor^tm2.1Ksvo/J
030206	RC::FPSit	R26 :: CAG	Synaptophysin / EGFP + tdTomato	Cre- and FLP-dependent ; xFP [synapse]	B6;129S6-Gt(ROSA)26Sor^{tm10(CAG-Syp/EGFP*,-tdTomato)Dym}/J
021875	Ai65D	R26 :: CAG	tdTomato	Cre- and FLP-dependent ; xFP	B6;129S-Gt(ROSA)26Sor^{tm65.1(CAG-tdTomato)Hze}/J
026932	RC::FLTG	R26 :: CAG	tdTomato ; EGFP	FLP-dependent tdT ; then Cre-dependent EGFP	B6.Cg-Gt(ROSA)26Sor^{tm1.3(CAG-tdTomato,-EGFP)Pjen}/J

Voltage Indicators : OptoPatch, CaViAr and ASAP2s					STRAIN NOMENCLATURE
028678	Floxopatch, Optopatch2	R26 :: CAG	CheRiff-eGFP :: QuasAr2-mOrange2	Cre-dependent ; all-optical electrophysiology	B6;129S6-Gt(ROSA)26Sor^tm1Acoh/J
029679	Optopatch3 Ai155	TIGRE :: TRE	CheRiff-eGFP :: QuasAr3-mCitrine	Cre- & Tet-dependent ; all-optical electrophysiology	B6;129S6-Igs7^{tm3(tetO-Optopatch3,CAG-tTA)Acoh}/J
031569	Ai169D	TIGRE :: TRE2 + CAG	ASAP2s + tTA2s	Cre- and Tet-dependent ; voltage indicator	B6.Cg-Igs7^{tm169.1(tetO-GFP/TPTE2,CAG-tTA2)Hze}/J

Chemogenetic Lines					STRAIN NOMENCLATURE
026220	R26-LSL-Gq-DREADD	R26 :: CAG	activ. Gq-DREADD (hM3Dq) :: mCitrine	Cre-inducible mCitrine ; then CNO-inducible canonical Gq pathway (burst firing)	B6N;129-Tg(CAG-CHRM3*,-mCitrine)1Ute/J
026943	RC::L-hM3Dq	R26 :: CAG	activ. Gq-DREADD (hM3Dq) / mCherry	Cre-inducible mCherry ; then CNO-inducible canonical Gq pathway (burst firing)	B6.Cg-Gt(ROSA)26Sor^{tm3.3(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen}/J
026219	R26-LSL-Gi-DREADD	R26 :: CAG	inhib. Gi-DREADD (hM4Di) :: mCitrine	Cre-inducible mCitrine ; then CNO-inducible canonical Gi pathway (neuron silencing)	B6N.129-Gt(ROSA)26Sor^{tm1(CAG-CHRM4*,-mCitrine)Ute}/J
029040	RC::FPDi	R26 :: CAG	inhib. Gi-DREADD (hM4Di) :: mCherry	FLP-inducible mCherry, then Cre- & CNO-inducible canonical Gi pathway (neuron silencing)	B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-mCherry,-CHRM4*)Dym}/J

Specific Promoter Lines					STRAIN NOMENCLATURE
007612	Thy1-ChR2-EYFP line 18	thymus cell antigen 1	ChR2 / EYFP	widespread brain ; opsin/xFP	B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615	Thy1-ChR2-EYFP line 9	thymus cell antigen 1	ChR2 / EYFP	widespread brain ; opsin/xFP	B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
014546	ChAT-ChR2(H134R)-EYFP line 6	choline acetyltransferase	mhChR2 / EYFP	cholinergic neurons ; opsin/xFP	B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J
014548	VGAT-ChR2(H134R)-EYFP	vesicular GABA transporter	mhChR2 / EYFP	GABAergic interneurons ; opsin/xFP	B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
021206	M72-IRES-ChR2-YFP	olfactory receptor 160	ChR2*H134R / EYFP	M72+ olfactory sensory neurons ; opsin/xFP	STOCK Olfr160^{tm1.1(COP4*/EYFP)Tboz}/J
024742	TRE-GCaMP6s line G6s2	TRE	GCaMP6s	Tet-inducible ; calcium indicator	B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
025111	Snap25-2A-GCaMP6s-D	synaptosomal-assoc. prot.25	GCaMP6s	widespread brain ; calcium indicator	B6.Cg-Snap25^tm3.1Hze/J
033342	Cdh5-GCaMP8	cadherin 5	GCaMP8	blood/brain barrier (endothelial cells)	B6;SJL-Tg(Cdh5-GCaMP8)B20-6Mik/J
030525	Thy1-jRGECO1a-WPRE, line GP8.20	thymus cell antigen 1	jRGECO1a	brain (denser cortex) ; calcium indicator [red]	STOCK Tg(Thy1-jRGECO1a)GP8.20Dkim/J
030526	Thy1-jRGECO1a-WPRE, line GP8.31	thymus cell antigen 1	jRGECO1a	brain (sparser cortex) ; calcium indicator [red]	STOCK Tg(Thy1-jRGECO1a)GP8.31Dkim/J
030527	Thy1-jRGECO1a-WPRE, line GP8.58	thymus cell antigen 1	jRGECO1a	brain ; calcium indicator [red]	C57BL/6J-Tg(Thy1-jRGECO1a)GP8.58Dkim/J
030528	Thy1-jRGECO1a-WPRE, line GP8.62	thymus cell antigen 1	jRGECO1a	brain ; calcium indicator [red]	STOCK Tg(Thy1-jRGECO1a)GP8.62Dkim/J
031478	PrismPlus	Cx3cr1 :: Snap25 :: Mobp :: Aldh1L1	EGFP :: YFP :: Cerulean :: DsRedMax	microglia :: neurons :: oligodendrocytes :: astrocytes	STOCK Cx3cr1^tm1Litt Tg(Prism)1989Htz/J
032188	mito::mKate2	CAG	mKate2	widespread fluorescence in mitochondria	STOCK Tg(CAG-mKate2)1Poche/J
022486	UBC PA-GFP	human ubiquitin C	PA-GFP	widespread photo-activatable xFP	B6.Cg-Ptprc^a Tg(UBC-PA-GFP)1Mnz/J

Thy1-GCaMP6 Lines from GENIE Project (Janelia/HHMI)					STRAIN NOMENCLATURE
024275	GP4.3	thymus cell antigen 1	GCaMP6s	high (hippocampus); mod (M1 S1 V1)	C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J
025776	GP4.12	thymus cell antigen 1	GCaMP6s	high in M1, amygdala, S1, hippocampus	C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J
024276	GP5.5	thymus cell antigen 1	GCaMP6f	high (hippocampus); mod (M1 S1 V1 amyg ob)	C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J
024339	GP5.11	thymus cell antigen 1	GCaMP6f	moderate in hippocampus, M1, S1, V1, olf. bulb	C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
025393	GP5.17	thymus cell antigen 1	GCaMP6f	high in hippocampus, M1, S1, V1, amygdala, olf. bulb	C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J