What We Do
Cellular Engineering (CE) provides custom-engineered human induced pluripotent stem cells (iPSCs). We apply and enhance cutting-edge CRISPR-based genome-editing technologies, implement improved iPSC handling methods and perform rigorous quality control. Our services focus on engineering precise base substitutions, gene knockouts/knockins, and genomic deletions with defined breakpoints in iPSCs as well as generating iPSC-derived models of multiple human cell types using established differentiation protocols. CE has deep expertise in disease modeling, null allele generation and other complex genome editing. We also offer project consultation, protocol training and reagents to support the use of human iPSCs by JAX investigators.
CE continually adapts to evolving trends by evaluating and adopting emerging technologies in genome engineering. We also engage in research and development (R&D) efforts to ensure the service remains at the forefront of iPSC-based research applications.
In addition, through a collaboration with Dr. Bill Skarnes, CE has a separate team funded by NIH and CZI to generate hundreds of single nucleotide variant, gene knockout, and gene-tagged iPSC lines for the iPSC Neurodegenerative Disease Initiative (iNDI). iNDI is a large-scale project that aims to generate a massive suite of engineered isogenic iPSC lines to support research across multiple dementia-related neurodegenerative disorders. Each line is then distributed to the research community.
Technologies and Services Offered
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Custom Genome Editing: Precision editing using CRISPR reagents, including introduction of disease-associated mutations and development of disease models, null allele generation (monoallelic and biallelic), and complex editing.
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iPSC Differentiation: Development and implementation of protocols to differentiate iPSCs into models of specific cell types, including monolayer-based neuronal (e.g., neural progenitor cells, mature neurons) and immune cells (e.g., dendritic cells). Differentiation methods include exposure to cell-signaling factors or inductive molecules and genomic engineering of inducible transcription factor expression.
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Genome-Wide SNP Array Analysis: Implementation of genome-wide SNP microarray analysis for comprehensive quality control analysis of copy number variation and loss of heterozygosity in pre- and post-edited human iPSC lines.
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Training and Consultation: Training on iPSC- and CRISPR-related protocols and operation of supporting instruments, such as the Bio-Rad Droplet Digital PCR (ddPCR) systems. Project consultation and advice are also offered.
