What We Do
We provide state-of-the-art instrumentation and expertise in flow cytometry and fluorescence-activated cell sorting (FACS). As a full-service core facility, we can prepare and run samples for our clients or train them in all aspects of running samples independently, including cell sorting. We offer in-depth consultation on high-parameter antibody panels and experimental design to ensure high-quality data.
Services and Technologies Offered
- >30-color capability for cell analysis on spectral flow cytometers
- Up to 28-color and 6-way cell sorting on BD FACSymphony S6 cell sorters
- Sterile sorting of cells into tubes or plates as needed for downstream applications
- Tissue dissociation into individual cells and sample preparation
- Cell staining with custom antibody panels
- Cytometric data acquisition
- Inventory of titrated monoclonal antibodies
- Data analysis with FlowJo or instrument vendor software
- Training in all aspects of sample preparation, staining, instrument use, and data analysis
Equipment
Bar Harbor
- BD FACSymphony S6 28-color, 5-laser, 6-way cell sorter
- BD FACSAria II, 18-color, 5-laser, 4-way cell sorter
- BD FACSymphony A5 SE 48-channel, 5-laser, spectral cytometer
- BD FACSymphony A5 28-color, 5-laser cytometer
- BD LSR II 18-color 4-laser Cytometer
- BD FACSCalibur 4-color, 2-laser cytometer
- Thermo Attune NxT 14-color, 4-laser cytometer*
- Thermo Attune NxT 12-color, 3-laser cytometer*
- Miltenyi GentleMACS Octo Dissociators with Heaters
*BSL2 compliant
Farmington
- FACSymphony S6 28-color, 5-laser, 6-way cell sorter*
- FACSAria Fusion 16-color, 4 laser, 4-way cell sorter*
- Cytek Aurora 64-channel, 5-laser, spectral cytometer*
- BD FACSymphony A5 28-color, 5-laser cytometer*
- BD LSR Fortessa X-20 16-color, 4-laser cytometer*
- BD Accuri 4-color, 2-laser cytometer*
- Intellicyt iQue Screener Plus, 4-laser, 13-color, plate-based screener*
*BSL2 compliant
