Immune Humanized Mouse Models

Humanized NSG mice are a therapeutically translational platform for the evaluation of human immune cells in vivo, the evaluation of single and combinatorial drug approaches, and the production of predictive data for the clinic. These models can be used in immuno-oncology applications, stem cell biology, and therapeutic efficacy/toxicity testing. With thousands of publications, the Jackson Laboratory's Humanized NSG Platform is your partner for translational research.

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CD34+ Humanized Mouse Model Comparison

Produced by engraftment of human cord blood derived hematopoietic stem cells (HSCs), these models yield stable multilineage immune cell development and enable long-term longitudinal studies. Customization and CD34+ HSC engraftment of other NSG and NRG variants are also available.

Cells Engrafted	NSG™ (705557)	NSG™-IL15 (703089)	NSG™-SGM3 (701362)	NSG™-SGM3-IL15 (703321)	NSG™-FLT3 (703367)	NSG™-FLT3-IL15 (703722)
T Cells
Treg
B Cells
NK Cells
Myeloid
Mast Cells
cDC
pDC

Abundance relative to NSG

Present, but immature

Analysis Pending

Strain Names	Human Gene Expression	Benefits
CD34+ Hu-NSG™ Stock Number: 705557 Naïve Strain Data Sheet	N/A	Multilineage engraftment Foundational model for the NSG platform with thousands of publications T cell-dependent inflammatory responses No donor cell immune reactivity towards host
CD34+ Hu-NSG-SGM3 Stock Number: 701362 Naïve Strain Data Sheet	KITL (SCF), IL3, GM-CSF	Faster immune cell repopulation Higher myeloid cell engraftment Enhanced AML engraftment High expression of mast cells
CD34+ Hu-NSG-IL15 Stock Number: 703089 Naïve Strain Data Sheet	IL15	Highest NK cell engraftment
CD34+ Hu-NSG-SGM3-IL15 Stock Number: 703321 Hu-NSG-SGM3-IL15 Data Naïve Strain Data Sheet	KITL (SCF), IL3, GM-CSF, IL15	Reconstitution of T cell, Treg, myeloid cell, and mature natural killer cell populations Enables evaluation of combinatorial therapeutics in concert with multiple cell lineages Enables evaluation of combinatorial therapies evaluating T cells, NK cells, and myeloid cells
CD34+ Hu-NSG-FLT3 Stock Number: 703367 Hu-NSG-FLT3 Data Naïve Strain Data Sheet	FLT3 Ligand	Enhanced reconstitution of T cells, Tregs, myeloid cells, and immature natural killer cells Highest expression of conventional and plasmacytoid dendritic cells More tumor infiltrating lymphocytes with CDX or PDX engraftments
CD34+ Hu-NSG-FLT3-IL15 Stock Number: 703722 Hu-NSG-FLT3-IL15 Data Naïve Strain Data Sheet	FLT3 Ligand, IL15	Enhanced reconstitution of T cells, Tregs, myeloid cells, and mature natural killer cells High expression of conventional and plasmacytoid dendritic cells Diversity of immune cell models enables evaluation of combinatorial therapies that target T cells, NK cells, and relevant tumor micronenvironment cells.

PBMC Humanized Mouse Model Comparison

Created with adult peripheral blood mononuclear cells, the PBMC Hu-models feature quick engraftment and enable short-term efficacy and toxicity studies with mature human T cells.

Strain Names	Human Gene Expression	Benefits
PBMC Hu-NSG™ Stock Number: 745557 Naïve Strain Data Sheet	N/A	Enables short-term studies requiring effector and memory T cell function Allows assessment of patient-specific and donor-to-donor immune responses to therapeutics
PBMC Hu-NSG™ MHC I/II DKO Stock Number: 742516 Naïve Strain Data Sheet	N/A	Significantly delayed onset of GvHD Allows assessment of patient-specific and donor-to-donor immune responses to therapeutics Enables testing of cytokine release and downstream toxicity assays

CD34 Humanization

PBMC Humanization

NSG Variants Portfolio

Housing & Breeding of NSG™ Mice

Comparing Immunodeficient Models

Confidently Evaluate Your Therapies In The Humanized NSG Platform

CD34+ Humanized NSG and NSG variant mice are ready to ship 8-12 weeks following engraftment. With our guarantees of at least 25% CD45 engraftment, researchers can be confident in the abundance of human immune cells for their therapeutic study. CD3+ T cells are a critical cell of interest in immuno-oncology and our off-the-shelf Humanized NSG mice produce high and stable levels of CD3+ T cells following engraftment.

Why would drug developers test their antibody drug candidates in humanized mice?

Discover the indispensable role of humanized mice in biopharmaceutical research and their crucial importance in accurately modeling human diseases and immune responses. Immune cell humanized mice recapitulate critical components of the human immune system such as cells, cellular receptors, protein, cell-cell interactions, and individual diversity across a variety of donors. Through the engineering of a humanized immune system, researchers can rapidly advance the development of targeted treatments, providing valuable insights across various medical conditions.

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